FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_25.35100000044666.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_25.35100000044666.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107603
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTC1386812.888116502328002No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGT21341.983216081336022No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCG3830.3559380314675242No Hit
TTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTCT3580.3327044784996701No Hit
ATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTCTTC2370.22025408213525643Illumina PCR Primer Index 3 (95% over 22bp)
TTCTCTTTCCCTTTCTTTCTCTTTCTCTTTCCCTTTTTCTCTTTGTGTCTT1790.16635223924983505No Hit
GTATGGAATTGAATGGAAACATTGAAAGGAATTGAATGGAATCATCATTGA1160.10780368577084283No Hit
GTTTCTTTTCGATTCGTTTTGATTCGATTCTTTTTGATTCATGTCGATTCG1120.10408631729598616No Hit
GGAATATCCTGTATTGCAACCAGTAACCAGAAGATATCATACACACTGTGT1100.10222763305855785No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCAA253.8672002E-545.00000421
GCTATAG207.0047873E-445.023
TAACGCT207.0047873E-445.015
CTTTGCG207.0047873E-445.035
GCAATGG207.0047873E-445.029
GTAACGC207.0047873E-445.014
TGCCGTC16550.042.28096845
AGCTTCC403.4194272E-739.37522
GCTTCCT403.4194272E-739.37523
CGTTTTG301.1330598E-437.49999615
ATGCCGT19250.036.2337744
CTATGTG250.002098649736.0000049
TCTTGTG250.002098649736.0000047
AACCGAT250.002098649736.00000442
TTGGAGT250.002098649736.0000046
CCGATTG250.002098649736.00000444
GAACTAG250.002098649736.00000417
ACCGATT250.002098649736.00000443
CAACCGA250.002098649736.00000441
CGATTGA250.002098649736.00000445
Produced by FastQC (version 0.11.2)