Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l02n01_bir_0113_pb_24.3510000004464d.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 22675 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 50 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC | 5561 | 24.524807056229328 | RNA PCR Primer, Index 23 (95% over 21bp) |
TCTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGT | 466 | 2.0551267916207276 | No Hit |
TTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTCT | 192 | 0.8467475192943771 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCG | 117 | 0.5159867695700111 | No Hit |
ATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTCTTC | 104 | 0.45865490628445427 | RNA PCR Primer, Index 23 (95% over 24bp) |
TATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTCTT | 49 | 0.2160970231532525 | RNA PCR Primer, Index 23 (95% over 23bp) |
TCTTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCG | 25 | 0.11025358324145534 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 700 | 0.0 | 40.82143 | 45 |
ATCTCGT | 760 | 0.0 | 37.894737 | 37 |
TCGTATG | 760 | 0.0 | 37.894737 | 40 |
TGATCTC | 755 | 0.0 | 37.847683 | 35 |
TTGATCT | 755 | 0.0 | 37.847683 | 34 |
TCTCCGA | 750 | 0.0 | 37.8 | 11 |
AGCCCAC | 765 | 0.0 | 37.647057 | 17 |
TCTCGTA | 765 | 0.0 | 37.647057 | 38 |
CGTATGC | 765 | 0.0 | 37.647057 | 41 |
CCGAGCC | 765 | 0.0 | 37.647057 | 14 |
TCCGAGC | 765 | 0.0 | 37.647057 | 13 |
CTCGTAT | 765 | 0.0 | 37.647057 | 39 |
CTCCGAG | 765 | 0.0 | 37.647057 | 12 |
GTTGATC | 760 | 0.0 | 37.598682 | 33 |
GTATGCC | 760 | 0.0 | 37.598682 | 42 |
GCCCACG | 760 | 0.0 | 37.598682 | 18 |
CACGAGA | 760 | 0.0 | 37.598682 | 21 |
CCCACGA | 760 | 0.0 | 37.598682 | 19 |
AGAGTTG | 760 | 0.0 | 37.598682 | 30 |
ACGAGAG | 760 | 0.0 | 37.598682 | 27 |