FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_24.3510000004464d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_24.3510000004464d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22675
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC556124.524807056229328RNA PCR Primer, Index 23 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGT4662.0551267916207276No Hit
TTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTCT1920.8467475192943771No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCG1170.5159867695700111No Hit
ATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTCTTC1040.45865490628445427RNA PCR Primer, Index 23 (95% over 24bp)
TATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTCTT490.2160970231532525RNA PCR Primer, Index 23 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCG250.11025358324145534No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC7000.040.8214345
ATCTCGT7600.037.89473737
TCGTATG7600.037.89473740
TGATCTC7550.037.84768335
TTGATCT7550.037.84768334
TCTCCGA7500.037.811
AGCCCAC7650.037.64705717
TCTCGTA7650.037.64705738
CGTATGC7650.037.64705741
CCGAGCC7650.037.64705714
TCCGAGC7650.037.64705713
CTCGTAT7650.037.64705739
CTCCGAG7650.037.64705712
GTTGATC7600.037.59868233
GTATGCC7600.037.59868242
GCCCACG7600.037.59868218
CACGAGA7600.037.59868221
CCCACGA7600.037.59868219
AGAGTTG7600.037.59868230
ACGAGAG7600.037.59868227