FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_23.35100000044623.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_23.35100000044623.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117298
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC2847724.277481286978464RNA PCR Primer, Index 23 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGT94758.077716585108014No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCG14531.2387252979590444No Hit
TTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTCT4610.3930160787055193No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCACA206.994934E-445.0162124
CTGTATG207.0096593E-444.9970179
TGCCGTC30650.043.52892745
CGTCTTC356.203254E-638.56886745
GAATCCA250.00210010135.9976115
ACTGTCA250.00210010135.997618
CCGTCTT401.549395E-533.7477644
ATGCCGT40450.032.98297544
TATGCCG42600.031.31834443
CGTATGC42850.031.1881341
GTATGCC42800.031.17199942
GATCTCG42700.031.13962236
TCGTATG42950.031.11551540
CACGAGA42900.031.09933521
CCACGAG42900.031.09933520
CGAGAGT42550.031.09076728
ACGAGAG42500.031.07440827
ACGAGAC42950.031.06313322
GACGAGA42550.031.03789126
AGACGAG42700.031.0342425
Produced by FastQC (version 0.11.2)