FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_22.35100000044609.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_22.35100000044609.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12070
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC203116.826843413421706RNA PCR Primer, Index 23 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGT4423.6619718309859155No Hit
TTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTCT760.6296603148301574No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCG690.5716652858326429No Hit
ATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTCTTC220.18227009113504555RNA PCR Primer, Index 23 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC2250.043.045
ATCTCCG2900.034.9137910
CATCTCC2900.034.913799
TCTCCGA2900.034.9137911
ATGCCGT2800.034.5535744
AGCCCAC2950.034.32203317
GCCCACG2950.034.32203318
CCACGAG2950.034.32203320
GAGCCCA2950.034.32203316
CACGAGA2950.034.32203321
CCCACGA2950.034.32203319
ACGAGAC2950.034.32203322
CGAGCCC2950.034.32203315
CCGAGCC2950.034.32203314
TCCGAGC2950.034.32203313
CTCCGAG2950.034.32203312
GAGTTGA2900.034.13792831
AGAGTTG2900.034.13792830
ACGAGAG2900.034.13792827
AGACGAG2900.034.13792825
Produced by FastQC (version 0.11.2)