H23LNBCXX_l02n01_bir_0113_pb_20.351000000445c4.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l02n01_bir_0113_pb_20.351000000445c4.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	204595
Sequences flagged as poor quality	0
Sequence length	51
%GC	39

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC	17863	8.730907402429189	RNA PCR Primer, Index 23 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGT	4781	2.3368117500427674	No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC	913	0.4462474645030426	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCG	651	0.3181895940761016	No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC	623	0.3045040201373445	No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA	500	0.24438524890637603	No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA	433	0.21163762555292165	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTCTGTCTCTTATACACATCTCCGA	427	0.20870500256604513	No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA	404	0.19746328111635184	No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	307	0.15005254282851485	No Hit
TTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTCT	305	0.14907500183288935	No Hit
AAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGC	302	0.1476086903394511	No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA	278	0.13587819839194507	No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT	260	0.12708032943131553	No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	255	0.12463647694225177	No Hit
AAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAG	254	0.12414770644443901	No Hit
GGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGAGC	245	0.11974877196412426	No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA	244	0.1192600014663115	No Hit
AAAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG	221	0.1080182800166182	No Hit
GTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGAGCCCACGA	217	0.10606319802536719	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTCTGTCTCTTATACACATCTCCGAG	215	0.1050856570297417	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TGCCGTC	1975	42.597412	45
ATGCCGT	2455	34.268795	44
CGAGACG	2525	33.683353	23
GATCTCG	2505	33.68282	36
CGAGAGT	2500	33.660183	28
AGACGAG	2510	33.615723	25
ACGAGAG	2510	33.615723	27
ACGAGAC	2545	33.595467	22
GAGACGA	2525	33.594246	24
GACGAGA	2505	33.593	26
ATCTCGT	2520	33.57161	37
AGAGTTG	2495	33.547276	30
CACGAGA	2545	33.507057	21
TCTCGTA	2525	33.505135	38
TGATCTC	2515	33.459427	35
CTCGTAT	2530	33.430744	39
TATGCCG	2520	33.384876	43
CGTATGC	2535	33.364803	41
CCACGAG	2560	33.310726	20
GTATGCC	2530	33.252922	42

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content