FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_19.351000000445ab.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_19.351000000445ab.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC68.955223880597014RNA PCR Primer, Index 23 (95% over 21bp)
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGTCCATTCTAA11.4925373134328357RNA PCR Primer, Index 27 (96% over 28bp)
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAACAAAGA11.4925373134328357RNA PCR Primer, Index 27 (96% over 28bp)
CTATAACAGGTAACAGTAGTGATTTCAAAAATTTTAATATCTCAATAGTTT11.4925373134328357No Hit
GAAGTTGGTTATCTAACGGACAATTATAAAACCAGATGTACTTGTAGCCTG11.4925373134328357No Hit
CTTTTCACCCTTCCCTCACGGTACTACTTCGCTATCGGTCACCTGTCTCTT11.4925373134328357No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACCTGTCT11.4925373134328357No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACCAAACACCTT11.4925373134328357Illumina PCR Primer Index 8 (96% over 27bp)
AAAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCC11.4925373134328357No Hit
TCTTGAATCTCTCCATACACAGGCAAAAATAAGTGTGTTACTTAACATCTG11.4925373134328357No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACTTAGGCCGT11.4925373134328357Illumina PCR Primer Index 8 (96% over 27bp)
CGTATAGGGTGTGACGCCTGCTCGGTGCCGGAAGATTAAATGATGGCTGTC11.4925373134328357No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAACAAAA11.4925373134328357RNA PCR Primer, Index 27 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACGAGCGTTTATCTCGTATGCCGTC11.4925373134328357No Hit
TTCTACTGCCATTCCCATAAAAGTAAGTAGTCTCATTTTTGCCATATTCTG11.4925373134328357No Hit
CTTATACACTTCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC11.4925373134328357RNA PCR Primer, Index 23 (95% over 21bp)
GATATTGATGGTGATGGTCAAGTAAACTATGAAGAGTTTGTACAAATGCTG11.4925373134328357No Hit
TGTCACTCCAATCCCCTACGATGTCGTCAACGATCTCAAAGGAAGCTATTA11.4925373134328357No Hit
GTGGTTACCCAGGTGTATACAATTATCAATACTCGTTCAACTGAAAACCTA11.4925373134328357No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTGATACCCCTTTTTCCC11.4925373134328357No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACCTGTCTCTTA11.4925373134328357No Hit
CGGGTGCACCGCCGACCGACCTTGATCTTCTGAGAAGGGTTCGAGTGTGAG11.4925373134328357No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATTTCGTATGCCGTC11.4925373134328357No Hit
ATGAACGACTTGCTGAAAGAAAGTTTTAAGACTGTCTCTTATACACATCTC11.4925373134328357No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGACTTGATCTCGTATGCCGTC11.4925373134328357No Hit
ATTTATCTCTATCCTTCCTCAAGTAGTACACCATTTGAACTGTCTCTTATA11.4925373134328357No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA11.4925373134328357No Hit
TCTGAAGCCTCTGTGATTGTAAGGGAACACCTGTCTCTTATACACATCTCC11.4925373134328357No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTT11.4925373134328357No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTTATCTCGTATGCCGTC11.4925373134328357No Hit
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACC11.4925373134328357No Hit
ATCCTTTCCCTCATCGAATCCAAGCCTCATCTGGGCCTGTCTCTTATACAC11.4925373134328357No Hit
CATACAGCCAAGCATTAACAAAAGCCAATAAAGGTCTTAACTGGGACAAAG11.4925373134328357No Hit
CTTAAAACCCCACAACCGGTTGTTTGAGGACAGTTTCCGAGCTCACATAAC11.4925373134328357No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACTGTGTCTCCTA11.4925373134328357RNA PCR Primer, Index 27 (96% over 28bp)
CTGTTGCCTCCCGTAGGAGTCTGGACCGTGTCCCCGTACCTGTCTCTTATA11.4925373134328357No Hit
CCTTTCGGCCTCGCCTTAGGGGTCGACTCACCCTGCCCCGATTAACGTTGG11.4925373134328357No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTTATCTCGTATGCCGTC11.4925373134328357No Hit
ATGGAGGGGGATAACTACTGGAAACGGTAGCTACTGTCGCTTATACACATC11.4925373134328357No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAGAAAATT11.4925373134328357RNA PCR Primer, Index 27 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGCCGAGCCTTGATCTCGTATGCCGTC11.4925373134328357No Hit
ATGCAGATAACATTAGTGTCTATATAGGGAATTCCCTGTCTCTTATACACA11.4925373134328357No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGCACCCCTA11.4925373134328357RNA PCR Primer, Index 27 (96% over 28bp)
AGCAATGGGTGTTTACAGCTCCATTTGGAATGCAGATGATTGGGCTACACA11.4925373134328357No Hit
TCTCTGTATAATACATCTTGAGACAGTCTTGAGAATCTACCTTGGAGAAAG11.4925373134328357No Hit
ACTCAAGACATTCCAGATGCTCGTGCATACTTGTTGAAGCTGTCTCTTATA11.4925373134328357No Hit
GGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGA11.4925373134328357No Hit
TTCCTCGAGAGCCTTAACTCTTACGTCCGTGAGAAAGAAGCGTCTATGATC11.4925373134328357No Hit
GCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGACTGATGGAG11.4925373134328357No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCCT11.4925373134328357No Hit
GTTTAGGGAAGGTCATTCAGTTGTGGTTGAAGGATTCATTAAGCCGTATAC11.4925373134328357No Hit
CCTTCGGCTCCCCTATTCGGTTAACCTTGCTACAGAATATAAGTCGCTGAC11.4925373134328357No Hit
GTCATAATGTTTTCATGCAATACATACTTGGTTCTCCAAATAACAACCTGT11.4925373134328357No Hit
CCTGTGGAGAGCAGAGAGCTGGCGGTACTGCCAGCAGCGGACCCTCAGAAG11.4925373134328357No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCG11.4925373134328357No Hit
CTCAAGTACAAGTCCAAAGTTTCACTGAAAGGCAATAGAGAAAGTGATTGA11.4925373134328357No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACACCGAGCCCA11.4925373134328357RNA PCR Primer, Index 27 (96% over 28bp)
ACGCTATCCAATGCCAAACTACGGCTATGTTGGTTGGTACGTTCAGGAGCT11.4925373134328357No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.4925373134328357No Hit
AAGTCTTTCTGGAGTCTTAACAAAGAGTATTATGGCCATTTAATAACCACT11.4925373134328357No Hit
GGTCGGGGACAGTGCATGGTAGGCAGTTTGACCTGTCTCTTATACACATCT11.4925373134328357No Hit
GAACATAACAAAGAAGTTCCAAAATGTTGACACTACGATGAATAATAGCTG11.4925373134328357No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCTC50.045.07
AATCTCT50.045.06
GTGTTAC50.045.035
ATAAGTG50.045.029
TTACTTA50.045.038
TAAGTGT50.045.030
GGCAAAA50.045.022
CTCCATA50.045.011
CTCTCCA50.045.09
AAATAAG50.045.027
ACATCTG50.045.045
TACACAG50.045.016
TGTTACT50.045.036
ACACAGG50.045.017
GCAAAAA50.045.023
AGTGTGT50.045.032
ACTTAAC50.045.040
TAACATC50.045.043
GAATCTC50.045.05
TCTTGAA50.045.01