FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_16.3510000004454e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_16.3510000004454e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25085
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC741929.57544349212677RNA PCR Primer, Index 45 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGT6982.782539366155073No Hit
TTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCT2330.9288419374127965No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCG2080.8291807853298784No Hit
ATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCTTC420.16743073549930237RNA PCR Primer, Index 45 (95% over 24bp)
TATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCTT350.1395256129160853RNA PCR Primer, Index 45 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCG260.1036475981662348No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC8000.041.062545
ATGCCGT8600.038.19767444
GCCCACG8850.037.6271218
TATGCCG8750.037.5428643
AGCCCAC8900.037.4157317
CACGAGA8900.037.4157321
CCCACGA8900.037.4157319
ACGAGAC8900.037.4157322
CGAGACG8900.037.4157323
CATTAAT8850.037.37288332
CGTATGC8850.037.37288341
TCGTATG8850.037.37288340
GTATGCC8800.037.32954442
AATCTCG8800.037.32954436
ATCTCGT8800.037.32954437
GACGTTC8800.037.32954426
TCATTAA8800.037.32954431
GAGACGT8800.037.32954424
TTCATTA8750.037.28571330
GTTCATT8750.037.28571329