Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l02n01_bir_0113_pb_16.3510000004454e.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 25085 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 49 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC | 7419 | 29.57544349212677 | RNA PCR Primer, Index 45 (95% over 21bp) |
TCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGT | 698 | 2.782539366155073 | No Hit |
TTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCT | 233 | 0.9288419374127965 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCG | 208 | 0.8291807853298784 | No Hit |
ATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCTTC | 42 | 0.16743073549930237 | RNA PCR Primer, Index 45 (95% over 24bp) |
TATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCTT | 35 | 0.1395256129160853 | RNA PCR Primer, Index 45 (95% over 23bp) |
TCTTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCG | 26 | 0.1036475981662348 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 800 | 0.0 | 41.0625 | 45 |
ATGCCGT | 860 | 0.0 | 38.197674 | 44 |
GCCCACG | 885 | 0.0 | 37.62712 | 18 |
TATGCCG | 875 | 0.0 | 37.54286 | 43 |
AGCCCAC | 890 | 0.0 | 37.41573 | 17 |
CACGAGA | 890 | 0.0 | 37.41573 | 21 |
CCCACGA | 890 | 0.0 | 37.41573 | 19 |
ACGAGAC | 890 | 0.0 | 37.41573 | 22 |
CGAGACG | 890 | 0.0 | 37.41573 | 23 |
CATTAAT | 885 | 0.0 | 37.372883 | 32 |
CGTATGC | 885 | 0.0 | 37.372883 | 41 |
TCGTATG | 885 | 0.0 | 37.372883 | 40 |
GTATGCC | 880 | 0.0 | 37.329544 | 42 |
AATCTCG | 880 | 0.0 | 37.329544 | 36 |
ATCTCGT | 880 | 0.0 | 37.329544 | 37 |
GACGTTC | 880 | 0.0 | 37.329544 | 26 |
TCATTAA | 880 | 0.0 | 37.329544 | 31 |
GAGACGT | 880 | 0.0 | 37.329544 | 24 |
TTCATTA | 875 | 0.0 | 37.285713 | 30 |
GTTCATT | 875 | 0.0 | 37.285713 | 29 |