FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_15.35100000044524.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_15.35100000044524.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40350
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC1426235.3457249070632RNA PCR Primer, Index 45 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGT444511.016109045848822No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCG12313.0508054522924413No Hit
TTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCT1070.265179677819083No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC14300.043.1118945
GCCGTCT601.4551915E-1037.49999645
ATGCCGT18650.033.056344
AATCTCG20000.031.0536
ATCTCGT20100.031.00746237
GAGCCCA20400.030.99264716
TAATCTC20050.030.97256935
AGCCCAC20350.030.9582317
TCTCGTA20150.030.9305238
AGACGTT20150.030.9305225
TTAATCT20150.030.9305234
GCCCACG20300.030.92364718
ACGAGAC20300.030.92364722
CGAGCCC20450.030.9168715
CATTAAT19950.030.90225632
GAGACGT20100.030.89552324
CCCACGA20250.030.8888919
CGAGACG20250.030.8888923
CCGAGCC20550.030.87591414
TCCGAGC20550.030.87591413
Produced by FastQC (version 0.11.2)