Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l02n01_bir_0113_pb_14.3510000004450a.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 24809 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 40 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC | 4712 | 18.993107340078197 | RNA PCR Primer, Index 45 (95% over 21bp) |
TCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGT | 1258 | 5.070740457092184 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCG | 170 | 0.6852351969043493 | No Hit |
TTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCT | 91 | 0.36680237010762223 | No Hit |
ATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCTTC | 28 | 0.11286226772542222 | RNA PCR Primer, Index 45 (95% over 24bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 450 | 0.0 | 44.498005 | 45 |
ATGCCGT | 580 | 0.0 | 34.524315 | 44 |
AATCTCG | 610 | 0.0 | 33.195232 | 36 |
ATCTCGT | 610 | 0.0 | 33.195232 | 37 |
CATTAAT | 610 | 0.0 | 33.195232 | 32 |
TCTCGTA | 610 | 0.0 | 33.195232 | 38 |
GACGTTC | 610 | 0.0 | 33.195232 | 26 |
AGACGTT | 610 | 0.0 | 33.195232 | 25 |
ATTAATC | 610 | 0.0 | 33.195232 | 33 |
CTCGTAT | 610 | 0.0 | 33.195232 | 39 |
GTATGCC | 605 | 0.0 | 33.09769 | 42 |
GCCCACG | 605 | 0.0 | 33.09769 | 18 |
TATGCCG | 605 | 0.0 | 33.09769 | 43 |
CCCACGA | 605 | 0.0 | 33.09769 | 19 |
ACGAGAC | 605 | 0.0 | 33.09769 | 22 |
TTCATTA | 615 | 0.0 | 32.925354 | 30 |
ACGTTCA | 615 | 0.0 | 32.925354 | 27 |
GAGCCCA | 615 | 0.0 | 32.925354 | 16 |
GTTCATT | 615 | 0.0 | 32.925354 | 29 |
TAATCTC | 615 | 0.0 | 32.925354 | 35 |