FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_14.3510000004450a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_14.3510000004450a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24809
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC471218.993107340078197RNA PCR Primer, Index 45 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGT12585.070740457092184No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCG1700.6852351969043493No Hit
TTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCT910.36680237010762223No Hit
ATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCTTC280.11286226772542222RNA PCR Primer, Index 45 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4500.044.49800545
ATGCCGT5800.034.52431544
AATCTCG6100.033.19523236
ATCTCGT6100.033.19523237
CATTAAT6100.033.19523232
TCTCGTA6100.033.19523238
GACGTTC6100.033.19523226
AGACGTT6100.033.19523225
ATTAATC6100.033.19523233
CTCGTAT6100.033.19523239
GTATGCC6050.033.0976942
GCCCACG6050.033.0976918
TATGCCG6050.033.0976943
CCCACGA6050.033.0976919
ACGAGAC6050.033.0976922
TTCATTA6150.032.92535430
ACGTTCA6150.032.92535427
GAGCCCA6150.032.92535416
GTTCATT6150.032.92535429
TAATCTC6150.032.92535435