FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_13.351000000444ef.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_13.351000000444ef.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100075
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC18521.8506120409692732RNA PCR Primer, Index 45 (95% over 21bp)
ATCCTACACAGATCGGTTCAAAGTCCAATGCAAAGCTACAGTAAAGGTTCA2190.2188358730951786No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA1330.13290032475643268No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGT1210.12090931801149137No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCG1190.11891081688733449No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1130.11291531351486385No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC2050.040.60975645
CATTAAT2300.038.15217232
GACGTTC2250.038.026
CGAGACG2250.038.023
AGCCCAC2350.037.34042717
CCCACGA2350.037.34042719
TAATCTC2350.037.34042735
AGACGTT2300.037.17391225
ATGCCGT2250.037.044
TTCATTA2400.036.562530
CCACGAG2400.036.562520
GTTCATT2350.036.3829829
GTATGCC2300.036.19565242
GAGACGT2400.035.62524
GCCCACG2550.035.29411718
TCATTAA2500.035.131
ACGTTCA2450.034.8979627
TATGCCG2400.034.687543
ATCTCGT2550.034.41176637
TTAATCT2550.034.41176634
Produced by FastQC (version 0.11.2)