H23LNBCXX_l02n01_bir_0113_pb_12.351000000444c5.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l02n01_bir_0113_pb_12.351000000444c5.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	308529
Sequences flagged as poor quality	0
Sequence length	51
%GC	40

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC	38977	12.633172246369062	RNA PCR Primer, Index 45 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGT	7497	2.4299174469822935	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCG	1392	0.4511731474188812	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	809	0.26221198007318597	No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	795	0.2576743191077662	No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC	739	0.2395236752460871	No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	623	0.20192591296118034	No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	596	0.1931747096707279	No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	595	0.19285059103034075	No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	544	0.17632054037059727	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	492	0.15946637107046663	No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	481	0.15590106602620823	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	457	0.14812221865691716	No Hit
TTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCT	445	0.14423279497227165	No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC	383	0.12413743926826976	No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	379	0.12284096470672125	No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	353	0.11441388005665593	No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA	341	0.1105244563720104	No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA	339	0.10987621909123615	No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	336	0.10890386317007478	No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA	331	0.10728326996813912	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTCGTC	20	7.026127E-4	44.998867	17
TGCCGTC	4285	0.0	43.528656	45
ATGCCGT	5055	0.0	36.898182	44
AGCCCTA	25	0.002104985	35.999096	28
AGACGTT	5245	0.0	35.60444	25
GACGTTC	5250	0.0	35.570534	26
CGAGACG	5270	0.0	35.563618	23
TATGCCG	5250	0.0	35.527676	43
CGTTCAT	5250	0.0	35.527676	28
GAGACGT	5270	0.0	35.520924	24
CGTATGC	5265	0.0	35.511925	41
CACGAGA	5285	0.0	35.505253	21
AATCTCG	5285	0.0	35.505253	36
CTCGTAT	5280	0.0	35.496265	39
GTATGCC	5255	0.0	35.493874	42
ACGAGAC	5295	0.0	35.4382	22
ACGTTCA	5265	0.0	35.426456	27
TCTCGTA	5315	0.0	35.38951	38
ATCTCGT	5310	0.0	35.338093	37
TCGTATG	5305	0.0	35.328987	40

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content