FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_10.35100000044482.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_10.35100000044482.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40739
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC1365733.52315962591128RNA PCR Primer, Index 45 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGT16984.167996268931491No Hit
ATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCTTC3780.9278578266525934RNA PCR Primer, Index 45 (95% over 24bp)
CTCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCG3240.7953067085593657No Hit
TTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCT2450.6013893320896438No Hit
TATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCTT1070.26264758585139547RNA PCR Primer, Index 45 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCG560.13746041876334716No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT510.1251871670880483No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTC453.6561687E-1045.045
TGCCGTC14800.042.11148545
ATGCCGT16750.037.20895444
CCGTCTT552.6120688E-936.8181844
CGAGACG17250.036.65217623
AGACGTT17150.036.603525
GAGACGT17150.036.603524
GTTCATT17100.036.5789529
GACGTTC17100.036.5789526
CGTTCAT17150.036.47230528
ACGTTCA17150.036.47230527
CCACGAG17350.036.4409220
CACGAGA17350.036.4409221
GCCCACG17300.036.41618318
CCCACGA17300.036.41618319
AATCTCG17200.036.3662836
ATTAATC17200.036.3662833
ACGAGAC17400.036.3362122
ATCTCGT17300.036.28612537
CGTATGC17300.036.28612541
Produced by FastQC (version 0.11.2)