FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_1.3510000004435d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_1.3510000004435d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35899
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTC827123.03963898715842No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGT7542.1003370567425277RNA PCR Primer, Index 44 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCG2460.6852558567091005No Hit
TTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCT2090.5821889189113902RNA PCR Primer, Index 44 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCTTC770.21449065433577535RNA PCR Primer, Index 37 (95% over 23bp)
TATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCTT590.16434998189364605RNA PCR Primer, Index 37 (95% over 22bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGAATT206.899696E-445.0558664
TGCCGTC9150.043.51784545
ATGCCGT10050.039.62072444
TCGTATG10450.038.3194240
CTCGTAT10450.038.3194239
TCTCGTA10400.038.28733438
CGTATGC10500.038.13694841
ATCTCGT10450.038.1041437
TATCTCG10450.038.1041436
GTATGCC10400.038.07102242
AGAATAT10550.037.95620332
TATGCCG10500.037.92269543
AGCCCAC10650.037.81104317
CCACGAG10650.037.81104320
CACGAGA10650.037.81104321
ACGAGAC10650.037.81104322
CTAGAAT10650.037.81104330
GAATATC10600.037.7771633
ATATCTC10600.037.7771635
GCCCACG10700.037.63435418