Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l02n01_bir_0113_pb_1.3510000004435d.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 35899 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 49 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTC | 8271 | 23.03963898715842 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGT | 754 | 2.1003370567425277 | RNA PCR Primer, Index 44 (95% over 21bp) |
CTCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCG | 246 | 0.6852558567091005 | No Hit |
TTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCT | 209 | 0.5821889189113902 | RNA PCR Primer, Index 44 (95% over 23bp) |
ATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCTTC | 77 | 0.21449065433577535 | RNA PCR Primer, Index 37 (95% over 23bp) |
TATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCTT | 59 | 0.16434998189364605 | RNA PCR Primer, Index 37 (95% over 22bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGGAATT | 20 | 6.899696E-4 | 45.055866 | 4 |
TGCCGTC | 915 | 0.0 | 43.517845 | 45 |
ATGCCGT | 1005 | 0.0 | 39.620724 | 44 |
TCGTATG | 1045 | 0.0 | 38.31942 | 40 |
CTCGTAT | 1045 | 0.0 | 38.31942 | 39 |
TCTCGTA | 1040 | 0.0 | 38.287334 | 38 |
CGTATGC | 1050 | 0.0 | 38.136948 | 41 |
ATCTCGT | 1045 | 0.0 | 38.10414 | 37 |
TATCTCG | 1045 | 0.0 | 38.10414 | 36 |
GTATGCC | 1040 | 0.0 | 38.071022 | 42 |
AGAATAT | 1055 | 0.0 | 37.956203 | 32 |
TATGCCG | 1050 | 0.0 | 37.922695 | 43 |
AGCCCAC | 1065 | 0.0 | 37.811043 | 17 |
CCACGAG | 1065 | 0.0 | 37.811043 | 20 |
CACGAGA | 1065 | 0.0 | 37.811043 | 21 |
ACGAGAC | 1065 | 0.0 | 37.811043 | 22 |
CTAGAAT | 1065 | 0.0 | 37.811043 | 30 |
GAATATC | 1060 | 0.0 | 37.77716 | 33 |
ATATCTC | 1060 | 0.0 | 37.77716 | 35 |
GCCCACG | 1070 | 0.0 | 37.634354 | 18 |