FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pa_96.35100000044333.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pa_96.35100000044333.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC2422.018348623853214No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGC21.834862385321101No Hit
ATGTACTTATGGGGAAACAAGCCCGAGTGTTTGAAGCTGAGACTCTCCCCA10.9174311926605505No Hit
GTAATATACTAGAGTTATAATAAATTGTTTACTCTGCCTCACCAAACACAT10.9174311926605505No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTT10.9174311926605505No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTACTCTG10.9174311926605505No Hit
TTACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCG10.9174311926605505No Hit
GGGTAAGACACTTAACGACAATTGCTCCAACCCAGTGGTCACTAATGGGTC10.9174311926605505No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTG10.9174311926605505No Hit
GCGTGCGCTTTACGCCCAATAAATCCGGACAACGCTTGCCACCTACGTATT10.9174311926605505No Hit
GAGTACGGGATGTCGACTTGGAGGTTGTGCCCTTGAGGCTGTCTCTTATAC10.9174311926605505No Hit
CTCTTATACACATCTCCGAGGCCCACGAGACTCGCTATGATCTCGTATGCC10.9174311926605505No Hit
CATATCCCCTTACCGACGCTTATCGCAGATTAGCACGTCCTTCATCGCCTC10.9174311926605505No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA10.9174311926605505No Hit
GTAAAGACCAAGTGCTTTGAAATGACGATTCCACAGTTTCAGAATTTCTAC10.9174311926605505No Hit
CATTATTTCTCATCAATTGATAAGTACTGTGGTAATGCAAATTACTGTCTC10.9174311926605505No Hit
TCTTCAAGACGAATCTTGTTTGTGGCATTGGACCCTCAACAGAATCTACCC10.9174311926605505No Hit
GTACCACTGCTTCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACGTC10.9174311926605505No Hit
AAATGACAGCTCCACTTGGCAAATAATAGCTGTTACTTGATGGTATCCAAG10.9174311926605505No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGGAATATCTCGTATGCCGTC10.9174311926605505No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA10.9174311926605505No Hit
CGTTCAAATGTATTTCTCTCCCTTAGAGGGACTATTCCAACATCACTCCTT10.9174311926605505No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGCTG10.9174311926605505No Hit
GATGCAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATCTGTCTCT10.9174311926605505No Hit
CCACACATCTGGGGCAGCCTTCCCTGGCAGGGGCCCTCGCTGTCTCTTATA10.9174311926605505No Hit
CTTCCTCAGTCTGGATGGCATGTTGGCAGCCCAGACGAAAAAGCCCCGTAC10.9174311926605505No Hit
TATACGCACTTTGTAAATTGCGTATAATATCTTGCTATTTTGCACTAAAAA10.9174311926605505No Hit
GGATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGA10.9174311926605505No Hit
GTACGGGGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAG10.9174311926605505No Hit
GGAATACATGATCAGACACATGGTGTTCCAAGTGACGGAACATTGCTCTAA10.9174311926605505No Hit
ATCAGATGCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAACA10.9174311926605505No Hit
AGCTGGTATCTTCGACTGATTTCAGCTCCACGAGCAAGTCGCTTCACCTAC10.9174311926605505No Hit
CCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCTGACCACCTGTGTCGG10.9174311926605505No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCTGT10.9174311926605505No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCAGTC10.9174311926605505No Hit
GAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTAC10.9174311926605505No Hit
GTACGGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT10.9174311926605505No Hit
GATGACCACTATTGTTGGACCAAGTCCCCTCCTTCAAGAAAATTTGAAAAT10.9174311926605505No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCAGGTAACGTCAATGAGCAAAGG10.9174311926605505No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCA10.9174311926605505No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATC10.9174311926605505No Hit
AGGTTGCAGTGAGCCGAGATTGCGCCACTGCACTCCAGCCTGGGCAACAGA10.9174311926605505No Hit
AAATCTATCTTTTATCTCACCGCAAGCTTTTAATTCATTTCCAACTATTAG10.9174311926605505No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTA10.9174311926605505No Hit
CCTTCGCAGGCTTACAGAACGCTCCCCTACCCAACCGGTCTCTTATACACA10.9174311926605505No Hit
GTACGGGGGGTATCAACGCAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.9174311926605505No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGGACTATCTCGTATGCCGTC10.9174311926605505No Hit
ATATTTGAGTCTTTGCTTCTAATTCTTTGGGGATATTATAATACCTGTCTC10.9174311926605505No Hit
CATCATAGGATTAGTTTTCCCTGTTCTTGAATTATATGTAAACGACTCATA10.9174311926605505No Hit
TTTTTTTTTTTGCGTTGATACCACCCCGTACTCCTGTCTCTTATACACATC10.9174311926605505No Hit
AGGTCGGGCTCATCGATCGCCCGTACCACCAGCGTCAGGACCCACTGACTG10.9174311926605505No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCACGCCT10.9174311926605505No Hit
GAATGCCAATATTGGTGATAAACTTCGTTTTCCGATTAATTATAATACGCT10.9174311926605505No Hit
GTTGCAATTATAGTCATCTGGTAGGGGAGAATACGTAGATTTATGACAAAC10.9174311926605505No Hit
GTTAGTATACCCTTTACTAAATGGTGAGGATAGCTGCCAAAATAATCAAAA10.9174311926605505No Hit
ATAACTATCTCAGAGGGTAGCTTTTGACAGTTAAATAAGATGTATGGAGAC10.9174311926605505No Hit
ACATTTGATTGATAATTGGGCTAGGTATAGAATCTTTGTCCTAACATCTTC10.9174311926605505No Hit
GGTCATTTCACAGAGGAGGACAAGGCTCCTATCACAAGCCTGTTGGGCAAG10.9174311926605505No Hit
GGAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCAGGAACGGCGCGC10.9174311926605505No Hit
GCCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCTG10.9174311926605505No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC10.9174311926605505No Hit
AAGGGTATCCAAATAAGAACAGAGGAAGTCAAAGTATCCTTGTTTGAAGAC10.9174311926605505No Hit
GCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGCCCCTGT10.9174311926605505No Hit
GAGTACTACCTAACCCTCGTGTCCTGGATCGTCAACAACGGCATCTGGGCG10.9174311926605505No Hit
ATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGACTGTCTCTTATA10.9174311926605505No Hit
TATAGATGTTGTCAAAGTCATCTTCTTTTACTAATCGATCATTTTCAGCAT10.9174311926605505No Hit
CACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCG10.9174311926605505No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGGCTTATCTCGTATGCCGTC10.9174311926605505Illumina PCR Primer Index 10 (95% over 22bp)
TTTCTATTCGAGTGTTTTTGATTTGAATTCCTTTCTTTTCGAATCGATTCG10.9174311926605505No Hit
GGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAATTTTCACGCTTTT10.9174311926605505No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC10.9174311926605505No Hit
TCTAAACACTAATGATATAGAACCACAGACAAATATCTGTCTCTTATACAC10.9174311926605505No Hit
TTTCTCAGCTCTTCCACCTTCGTAGAGACAACATGAGAATGCCACCATGTC10.9174311926605505No Hit
AGATTCCTGAGGGTGGGTTTTTGTGTGATTCATCAGTATATACCTGTCTCT10.9174311926605505No Hit
CATATACACCATGGAATATTATGCAGCCATAAAAAATGATGAGTTCATGTC10.9174311926605505No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT10.9174311926605505No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.9174311926605505No Hit
TGGTATCAACGCAGAGTACGGGCAGTGGTATCAACCTGTCTCTTATACACA10.9174311926605505No Hit
CCCCATGAGTCTACCGGCAGAGTTCCGGTACGCATACATGCTGTCTCCTAT10.9174311926605505No Hit
GCGTTGATACCACTGCCCGTACTCTGCGTTGATACTGTCTCTTATACACAT10.9174311926605505No Hit
CAGTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC10.9174311926605505No Hit
ACACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATCACC10.9174311926605505No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAGAACC10.9174311926605505No Hit
ATCTTACATTATGCAGAACTATTTTTGTACAAATTGTTCTGTCTCTTATAC10.9174311926605505No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGC10.9174311926605505No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers