FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pa_95.35100000044326.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pa_95.35100000044326.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22324
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC392417.57749507256764No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGT5372.40548288837126No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCG1200.5375380756136893No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4000.043.87545
CGAGCCC4750.037.4210515
AGCCCAC4700.037.34042717
GCCCACG4700.037.34042718
GAGCCCA4700.037.34042716
ATGCCGT4700.037.34042744
TCTCCGA4850.037.11340311
TCCGAGC4850.037.11340313
CCGAGCC4900.036.73469514
CTCCGAG4900.036.73469512
CCACGAG4800.036.562520
CACGAGA4800.036.562521
CCCACGA4800.036.562519
ACGAGAC4800.036.562522
CGAGACC4800.036.562523
GAGACCC4800.036.562524
GTATGCC4750.036.47368242
GATAATC4750.036.47368233
ATAATCT4750.036.47368234
GGATAAT4750.036.47368232
Produced by FastQC (version 0.11.2)