FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pa_93.351000000442e1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pa_93.351000000442e1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences703531
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC65570.9320129461246199No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC14890.21164667939294785No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG10980.15606988178203945No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGT9490.13489099982801042No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA8110.11527565949474862No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC7770.11044289448510441No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTC7340.10433086814937793No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGC7130.10134592505518591No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATTAA301.1346909E-437.526791
ATGCCGT8550.037.1027444
GCCCACG8850.036.35346218
TATGCCG8750.036.25468443
CCCACGA9000.035.74757419
CGTATGC9050.035.5500741
TCGTATG9000.035.49759340
CTGGATA9100.035.3547430
GAGACCC9150.035.1615524
CTCGTAT9150.034.91566539
GTATGCC9150.034.66977742
CACGAGA9450.034.52146521
CCTGGAT9450.034.04530729
TGCCGTC9550.033.92439745
GATAATC9500.033.39246733
GGATAAT9600.033.2789932
TGGATAA9700.033.1678531
TAATCTC9950.033.01283335
ACGAGAC10000.032.84776722
AATCTCG9900.032.7250536