FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pa_90.35100000044277.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pa_90.35100000044277.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20851
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC674132.32938468178984No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGT6873.2948060045081773No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCG2451.1750035969497867No Hit
ATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCTTC780.37408277780442184Illumina PCR Primer Index 7 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCG320.15346985756078843No Hit
CTTAATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGT230.1103064601218167No Hit
TATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCTT220.10551052707304207Illumina PCR Primer Index 7 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC7050.044.36170645
ATGCCGT7550.041.4238444
GCCCACG7850.039.84076718
CCACGAG7850.039.84076720
TATGCCG7850.039.84076743
TCTCGTA7850.039.84076738
ACGAGAC7850.039.84076722
CGAGACC7850.039.84076723
AGACCCT7850.039.84076725
CTCGTAT7850.039.84076739
GAGACCC7850.039.84076724
AATCTCG7800.039.80769336
GATAATC7800.039.80769333
ATAATCT7800.039.80769334
GGATAAT7800.039.80769332
TGGATAA7800.039.80769331
AGCCCAC7900.039.58860817
CACGAGA7900.039.58860821
CCCACGA7900.039.58860819
CCGAGCC7900.039.58860814