Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l02n01_bir_0113_pa_90.35100000044277.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 20851 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 52 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC | 6741 | 32.32938468178984 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGT | 687 | 3.2948060045081773 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCG | 245 | 1.1750035969497867 | No Hit |
ATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCTTC | 78 | 0.37408277780442184 | Illumina PCR Primer Index 7 (95% over 22bp) |
TCTTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCG | 32 | 0.15346985756078843 | No Hit |
CTTAATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGT | 23 | 0.1103064601218167 | No Hit |
TATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCTT | 22 | 0.10551052707304207 | Illumina PCR Primer Index 7 (95% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 705 | 0.0 | 44.361706 | 45 |
ATGCCGT | 755 | 0.0 | 41.42384 | 44 |
GCCCACG | 785 | 0.0 | 39.840767 | 18 |
CCACGAG | 785 | 0.0 | 39.840767 | 20 |
TATGCCG | 785 | 0.0 | 39.840767 | 43 |
TCTCGTA | 785 | 0.0 | 39.840767 | 38 |
ACGAGAC | 785 | 0.0 | 39.840767 | 22 |
CGAGACC | 785 | 0.0 | 39.840767 | 23 |
AGACCCT | 785 | 0.0 | 39.840767 | 25 |
CTCGTAT | 785 | 0.0 | 39.840767 | 39 |
GAGACCC | 785 | 0.0 | 39.840767 | 24 |
AATCTCG | 780 | 0.0 | 39.807693 | 36 |
GATAATC | 780 | 0.0 | 39.807693 | 33 |
ATAATCT | 780 | 0.0 | 39.807693 | 34 |
GGATAAT | 780 | 0.0 | 39.807693 | 32 |
TGGATAA | 780 | 0.0 | 39.807693 | 31 |
AGCCCAC | 790 | 0.0 | 39.588608 | 17 |
CACGAGA | 790 | 0.0 | 39.588608 | 21 |
CCCACGA | 790 | 0.0 | 39.588608 | 19 |
CCGAGCC | 790 | 0.0 | 39.588608 | 14 |