FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pa_9.3510000004385b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pa_9.3510000004385b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences453914
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC65711.447631049053345TruSeq Adapter, Index 12 (95% over 21bp)
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG11430.25180981419387816No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGC9920.21854360077018997No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTC9650.2125953374427755No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC8600.18946320228060823No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGT8430.18571799944482875No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGGA8060.17756667562577932No Hit
GTCAAAGAGTGCTTGAAATTGTCGGGAGGGAAGCGGATGGGGGCCGGCGAT7600.16743259736425842No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGCT7330.16148433403684398No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTCT7130.15707821305357403No Hit
GCCTAAGCCCGGGCTTTTGATACGCTTGTGGAGACGTCGCTGCCGTGATCG6240.13747097467802272No Hit
CCCTTAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGACG6030.13284454764558926No Hit
CCGTTAGGCGCGTGCTGCAGACCACGATCACGGCAGCGACGTCTCCACAAG6020.13262424159642575No Hit
ACGTTAGGGAGTCCGGAGACGTCGGCGGGGGCCTCGGGAAGAGTTATCTTT5940.13086179320311778No Hit
GGCTTAGGCCGCCACCGTAATCCGCGTCGGTCCACGCCCCGAATCGATCGG5920.13042118110479076No Hit
CTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTTG5880.12953995690813677No Hit
GTGCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGATTAA5830.12843842666231928No Hit
CTTGTGGAGACGTCGCTGCCGTGATCGTGGTCTGCAGCACGCGCCTAACGG5780.1273368964165018No Hit
GCGCTTAAGCGCGCGACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTC5560.12249016333490485No Hit
CTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTA5380.11852465444996188No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA5350.11786373630247139No Hit
GTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCTT5330.1174231242041444No Hit
GGCTTTACCCCGCTCAGGCATAGTTCACCATCTTTCGGGTCCCGACAGGCA5310.11698251210581741No Hit
GGGTAAAGCCAGAGGAAACTCTGGTGGAAGCCCGCAGCGATACTGACGTGC5300.11676220605665391No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCG5300.11676220605665391No Hit
TGCCTACATTGTTCCATCGACCAGAGGCTGTTCACCTTGGAGACCTGATGC5110.11257639112254746No Hit
ATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCTCGG5100.11235608507338395No Hit
CTCCTACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCGC5080.11191547297505694No Hit
GGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGAATCGAGAGCTC4990.10993271853258547No Hit
GAGCAATCCGGTCCGCCGATCGATTCGGGGCGTGGACCGACGCGGATTACG4890.10772965804095049No Hit
GTCTTGAAACACGGACCAAGGAGTCTGACATGTGTGCGAGTCAACGGGTGA4860.10706873989346001No Hit
CCCTGGAAACGGCTCAGCCGGAGGTAGGGTCCAGCGGCTGGAAGAGCACCG4840.106628127795133No Hit
CTCCACAAGCGTATCAAAAGCCCGGGCTTAGGCCGCCACCGTAATCCGCGT4770.1050859854509885No Hit
GATTACGGTGGCGGCCTAAGCCCGGGCTTTTGATACGCTTGTGGAGACGTC4710.10376414915600751No Hit
CCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCTT4710.10376414915600751No Hit
ACCGTAATCCGCGTCGGTCCACGCCCCGAATCGATCGGCGGACCGGATTGC4710.10376414915600751No Hit
GTCTTAATCGACCAACACCCTTTGTGGGTTCTAGGTTAGCGCGCAGTTGGG4650.10244231286102655No Hit
GGCCTAAGTTCCCTGGAAAGGGGCGCCAGAGAGGGTGAGAGCCCCGTCGTG4640.10222200681186304No Hit
CTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAAC4580.10090017051688205No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT4570.10067986446771855No Hit
GAGTACGACCGGGCGTGAGCGGCACTCGGTCCTCCGGATTTTCAAGGGCCG4550.10023925236939156No Hit
CTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCGA4540.10001894632022806No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTTT253.8879618E-544.99823813
CGTATGC9000.036.7485641
ATGCCGT9000.036.7485644
TCGTATG8950.036.70247340
GTATGCC9100.036.3447342
CTCGTAT9050.036.2969239
TATGCCG9150.036.14612643
TCTCGTA9100.036.0974938
TATAACG250.002102758536.010492
CTACTTG9300.035.8050528
GACTACT9300.035.8050526
CCCACGA9350.035.6135819
CTTGCAA9300.035.5631231
CACGAGA9450.035.23671721
GAGACTA9450.035.23671724
TGCAATC9500.035.0512633
GCCCACG9550.034.86774418
CGAGACT9550.034.86774423
TTGCAAT9500.034.81442632
ACTACTT9600.034.68614227
Produced by FastQC (version 0.11.2)