H23LNBCXX_l02n01_bir_0113_pa_89.3510000004426a.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l02n01_bir_0113_pa_89.3510000004426a.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1846
Sequences flagged as poor quality	0
Sequence length	51
%GC	53

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC	317	17.172264355362945	No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGT	16	0.8667388949079089	No Hit
CTTATATACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC	10	0.5417118093174431	No Hit
TTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCT	10	0.5417118093174431	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCG	8	0.43336944745395445	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCG	8	0.43336944745395445	No Hit
CTTATACAATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCT	7	0.37919826652221017	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCG	6	0.3250270855904659	No Hit
CTTATAACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGT	5	0.27085590465872156	No Hit
CTTATACATATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC	4	0.21668472372697722	No Hit
CTTATACCATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCT	4	0.21668472372697722	No Hit
ATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCTTC	4	0.21668472372697722	Illumina PCR Primer Index 7 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGGC	4	0.21668472372697722	No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCCCGTATGCCGTC	4	0.21668472372697722	No Hit
ATTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC	4	0.21668472372697722	No Hit
ATGAAGACCAGGCGCACGCCGCGCCGGGGCAGGGTGATGGTTTCCAGCTCC	3	0.16251354279523295	No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGGATCATCTCGTATGCCGTC	3	0.16251354279523295	No Hit
CTTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGT	3	0.16251354279523295	No Hit
CTTATACACATCTCCGAGCCCACAGACCCTGGATAATCTCGTATGCCGTCT	3	0.16251354279523295	No Hit
GTGTAAAGTGCGCGCATGGGTCAGTTTCCTTATGCCGGAACGTCGGCGAGC	3	0.16251354279523295	No Hit
CTTATGCACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC	3	0.16251354279523295	No Hit
CTTATACACATCTCCGCGCCCACGAGACCCTGGATAATCTCGTATGCCGTC	3	0.16251354279523295	No Hit
CTTATAACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCT	3	0.16251354279523295	No Hit
CTTATACACAACTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC	3	0.16251354279523295	No Hit
CTTATACACATCTCCGAACCCACGAGACCCTGGATAATCTCGTATGCCGTC	3	0.16251354279523295	No Hit
CTTATACTCATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC	2	0.10834236186348861	No Hit
TTAAAGATACCCACCCGAAATTTCCTGAAGGTGGAAAAATGTCCCAAATAC	2	0.10834236186348861	No Hit
CTTATACACATCTCCGAGCCCAAGAGACCCTGGATAATCTCGTATGCCGTC	2	0.10834236186348861	No Hit
GATTGGATGTGACGGTCGTTGTGGACCAGCAGTCTTCACGACGCGTCGAAG	2	0.10834236186348861	No Hit
GAACATCGGCTCGTAGACCTTGTGGAACATCTCGGGCTTGACGCTGGCCTT	2	0.10834236186348861	No Hit
GGCTGAAGCTGCGGGACCGTCACCCGAACCTGCTCGACGAGATGCGCGCCA	2	0.10834236186348861	No Hit
GGGTAATCACGGTGGTTGCGAAAAAGTTTGAGTTTGTGCCAGGCGAGATTC	2	0.10834236186348861	No Hit
CTTATACGCATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC	2	0.10834236186348861	No Hit
GGCTTTAAGAGGCCATGTTGTAACAGGTGAGTTATAACAGGCTTTAACCCT	2	0.10834236186348861	No Hit
CCTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGT	2	0.10834236186348861	No Hit
CTTATACACATCTCCGAGCCCACGGACCCTGGATAATCTCGTATGCCGTCT	2	0.10834236186348861	No Hit
CTTATACACATCTCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCT	2	0.10834236186348861	No Hit
ACCGTGCCACGCGCCTGACGGTGCGGGACAACGGCACGGGTTTTGACGTGG	2	0.10834236186348861	No Hit
GTAGAGCCAATTGAAATCGATATTACAAACAAAAAAAGATATAGATATGTA	2	0.10834236186348861	No Hit
CTTATACAGATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC	2	0.10834236186348861	No Hit
CTTAAACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC	2	0.10834236186348861	No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	2	0.10834236186348861	No Hit
CTTATACACATCTCCGAGCCCACGAGACCATGGATAATCTCGTATGCCGTC	2	0.10834236186348861	No Hit
CTTATACACATCCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCT	2	0.10834236186348861	No Hit
GGACAAGTCAAGTCAGTGAGATGTAAGATTGGGGCCTTGAGGAGGGCCTGG	2	0.10834236186348861	No Hit
CTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCT	2	0.10834236186348861	No Hit
ACATTCACAATCTCTCCGGCATCGTGCTGGTGATGTGGATGCTGGTTGTCG	2	0.10834236186348861	No Hit
CTCATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC	2	0.10834236186348861	No Hit
ACCCGCCACCACGATGCTGCCGCCGGCAGCGCCCGCGCCTGGCCCTACGTC	2	0.10834236186348861	No Hit
CTTATACACATTCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGT	2	0.10834236186348861	No Hit
ATGGTGGGCTGCTCTCCGAGGCTGGCGTACCGGCCGTGGCCGACCCCGGCG	2	0.10834236186348861	No Hit
CCAGGAAGACGATGCGCTCGCGCAGCAGACGGGAGTAGATGTCGTAGGCGC	2	0.10834236186348861	No Hit
GTTGCTGCACCTGCCAGAGCTGGAGCCGTGGCCCCCAATGATGTCATCGTC	2	0.10834236186348861	No Hit
CTTTTACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC	2	0.10834236186348861	No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGAC	2	0.10834236186348861	No Hit
CTGTGGTGGTGGTCGCATTGCTCTTCGCCATGCTCATGCTGCGCTCCTTTG	2	0.10834236186348861	No Hit
CTTGTACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC	2	0.10834236186348861	No Hit
CTTATACACATCTCCGAGCCCCACGAGACCCTGGATAATCTCGTATGCCGT	2	0.10834236186348861	No Hit
GCACGTGGCCGCCTGGCTGCACGACTGCGGCAAGGTGACGACGCCCGAATT	2	0.10834236186348861	No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTTGATAATCTCGTATGCCGTC	2	0.10834236186348861	No Hit
CTTATACACACTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCT	2	0.10834236186348861	No Hit
CGTCTACAACGGCACGACGCAGGCCGTGATGATGGCGACGCCCGCGGATCT	2	0.10834236186348861	No Hit
TATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCTT	2	0.10834236186348861	Illumina PCR Primer Index 7 (95% over 21bp)
CTTATACCACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGT	2	0.10834236186348861	No Hit
CTTATACACATATCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC	2	0.10834236186348861	No Hit
ATCAACAGTGGTCCACGTGAAGATTCCACTCGTATTGGTCGTGCGGGTACA	2	0.10834236186348861	No Hit
CACCCATGCGGTGCATGGGCATGCCTTGCGGGCCATCGGACGGCGCAGGCG	2	0.10834236186348861	No Hit
GTCCGAGAAGGTGCAGCGCTGGCACGACAAGGTTCTGCTCAAGCTACCGAT	2	0.10834236186348861	No Hit
CTTATCACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCT	2	0.10834236186348861	No Hit
ATTGGAGGTGGAGCAGATGCCCAATCATCAACAGCAGGAATGTGGCCAGCT	2	0.10834236186348861	No Hit
CTTATACACTCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCT	2	0.10834236186348861	No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATATCGTATGCCGTC	2	0.10834236186348861	No Hit
GAATTTACTAGTCATGAACTTCGGACAGAATTAGAAAAGCAAATGGCAAAT	2	0.10834236186348861	No Hit
CTTAACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCT	2	0.10834236186348861	No Hit
CTTAATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGT	2	0.10834236186348861	No Hit
AGATAGGACCTGTGTTTACGATTCTAGAGATGATGAAATATCAGCAGTAGC	2	0.10834236186348861	No Hit
CGCGGCGAGCGCGAGGGCCTGTTCCATGGCGGTGTAGTCAGCGGCGGAAAA	2	0.10834236186348861	No Hit
TTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC	2	0.10834236186348861	No Hit
CTTATACACATCTCCGAGCCCACGAACCCTGGATAATCTCGTATGCCGTCT	2	0.10834236186348861	No Hit
AGGTCGAACCCCCCGCCCATCAGAAGTGCGGGAAGGGCGTTCTGTTCGTTG	2	0.10834236186348861	No Hit
ATATCTTCAATATCTTGTAATTCTTCTTTTTTGGTTTTATAACTTCTGTAC	2	0.10834236186348861	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCCGTC	45	6.7921064E-9	40.0	45
TATACAC	65	7.2759576E-11	34.615383	3
TTATACA	65	7.2759576E-11	34.615383	2
CTTATAC	65	7.2759576E-11	34.615383	1
TACACAT	60	1.7371349E-9	33.750004	5
ATACACA	60	1.7371349E-9	33.750004	4
ATCTCCG	55	4.0114173E-8	32.727272	10
CACATCT	55	4.0114173E-8	32.727272	7
CATCTCC	55	4.0114173E-8	32.727272	9
ACATCTC	55	4.0114173E-8	32.727272	8
ACACATC	55	4.0114173E-8	32.727272	6
ATGCCGT	55	4.0114173E-8	32.727272	44
AGCCCAC	60	8.646566E-8	30.000002	17
GTATGCC	60	8.646566E-8	30.000002	42
AATCTCG	60	8.646566E-8	30.000002	36
ATCTCGT	60	8.646566E-8	30.000002	37
GCCCACG	60	8.646566E-8	30.000002	18
CCACGAG	60	8.646566E-8	30.000002	20
TATGCCG	60	8.646566E-8	30.000002	43
GATAATC	60	8.646566E-8	30.000002	33

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content