FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pa_88.35100000044241.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pa_88.35100000044241.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC1016.129032258064516No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT58.064516129032258No Hit
GCCTAAAGGAGATAACATTTATGAATGGAGATCAACTATACTTGGCCCACC11.6129032258064515No Hit
GAAATATATTGATAGCAGAGAAAGACTCTTCATACCTGTTTCAGAACTCAA11.6129032258064515No Hit
CTTATACACATCTCCGAGCCCACGAGACAACTTAATATCTCGTATGCCGTC11.6129032258064515No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCG11.6129032258064515No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGGG11.6129032258064515No Hit
CCCTTGCACTGTATACACACAACACACGACAAACTATTCTAACGCTGTCTC11.6129032258064515No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAC11.6129032258064515No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTAA11.6129032258064515No Hit
ATCCCTTTTATGGCATTGTGATACTACAGTTGGGAATTTACACAAACAGAC11.6129032258064515No Hit
TTCTAAAGTTTATGTGGAGAGGCAGAAGACCTAGAATAGTCAATCAAATAT11.6129032258064515No Hit
GTACGGGATACCAGCTGGCTGCAACTGTTTATTAAAAACACAGCACTGTGC11.6129032258064515No Hit
GAGTTCTTTTTTCTTTTTTGTTTTTTTTTTTTTTGGTATTCTACCCAGATT11.6129032258064515No Hit
CTGTCTATAAACATCATGTCAAGAAGTCATCACATTTCTTTTGTTACACAG11.6129032258064515No Hit
TCTTATACACATCTCCGAGCCCACGAGCGACGCTATATCTCGTATGCCGTC11.6129032258064515No Hit
GTGTGGTTCTTTGACTTTGCCATACTGTTGCCGAGATTATAACCTATCTCG11.6129032258064515No Hit
GTAGAAGAACAAATCCATCGAGAGAACGATTGGAACTAGATAACCTGTCTC11.6129032258064515No Hit
CAGATGATTTGTCAATTGGAACATCGACGGAGTATCTTGTTGGCATCAGGT11.6129032258064515No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTAAGCCGT11.6129032258064515No Hit
CTTATACACATCTCCGAGCCCACGAGACTACAGACCATCTCGTATGCCGTC11.6129032258064515TruSeq Adapter, Index 7 (95% over 21bp)
AACCAGTACCGTGAGGGAAAGGCGAAAAAAAAAAAAAAAAAAAAAAAAGTA11.6129032258064515No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCT11.6129032258064515No Hit
ACGCAGAGTACTTTTTTTTTTTTCACCCAGAGTACTTTTTTTCTGTCTCTT11.6129032258064515No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTGTTTTGTTACCCTTTTCCGCCCCC11.6129032258064515No Hit
ATCTACCTCGAATACCCTTGACAGCGATAATGTAAAGGTTCTTTGCACCTG11.6129032258064515No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC11.6129032258064515No Hit
GACCGAGGAAGATGAGGAGAACAAGCCTAGTGTCATCGAAAAGCTTCACCG11.6129032258064515No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTATAATATCTCGTATGCCGT11.6129032258064515No Hit
GGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGA11.6129032258064515No Hit
CTTATACACATCTCCGAGCCCACGAGACTACATCATATCTCGTATGCCGTC11.6129032258064515No Hit
TCTTATACACATCTCCGAGCCCACGAGACAAAAGAAAATCTCGTATGCCGT11.6129032258064515No Hit
CTTATACACATCTCCGAGCCCACGACTAGATCCTATCTCGTATGCCGTCTT11.6129032258064515TruSeq Adapter, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTACGTTATATCTCGTATGCCGTC11.6129032258064515No Hit
ACTCACCTGACTGTTAGTTGGCTTTTTTTTTTTTTAAGAGCATTTTTCCTG11.6129032258064515No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAGCGGTCCCT11.6129032258064515No Hit
CTTATACACATCTCCGAGCCCACGAGACTACATAATATCTCGTATGCCGTC11.6129032258064515RNA PCR Primer, Index 44 (95% over 21bp)
GTTCATGTTCTTGTCCCTATGGGATTTGTCATTTGATGTTATTTAGACAGA11.6129032258064515No Hit
GTACTTTTTTTTTTTTTTTTTTCCGTTGATTCCACCCGCTGTCTCTTATAC11.6129032258064515No Hit
AAGCAGTGGTACCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAGTACCCCC11.6129032258064515No Hit
GAGCACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA11.6129032258064515No Hit
ATAAAAGAGCAAGAGACGGGCAATTATTGGGAAGTAGCAGCTTAACGATAA11.6129032258064515No Hit
CTTATACACATCTCCGAGCCCACGAGAGTACTTGCAATCTCGTATGCCGTC11.6129032258064515Illumina PCR Primer Index 12 (95% over 21bp)
GGCATGGTGGCACACCTGTAGTCCCAGCTACTTGGGACGCTGAGGTAGCAG11.6129032258064515No Hit
GCGTTGATACCACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCGC11.6129032258064515No Hit
GAGTCGGGAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGAC11.6129032258064515No Hit
ATGTAGAAGTGTTGAATCATTATATTCCTGTCTCTTATACACATCTCCGAG11.6129032258064515No Hit
CTTGGCAACCATCTTTTGATGGGAGTCCGGCTGTTCGATAGTCGGCCAAGG11.6129032258064515No Hit
ATTACGGATGATTCCATTCCATTGCATTCCATTCCATTCCATTCCTGTCTC11.6129032258064515No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAAGT50.045.04
TGTTGAA50.045.010
GTAGAAG50.045.03
GTTGAAT50.045.011
GTGTTGA50.045.09
TATATTC50.045.021
ATCTCCG50.045.043
CACATCT50.045.040
AGAAGTG50.045.05
TATACAC50.045.036
AATCATT50.045.015
TGTCTCT50.045.029
CCTGTCT50.045.027
GAAGTGT50.045.06
CATCTCC50.045.042
CTCTTAT50.045.032
TTATATT50.045.020
ACATCTC50.045.041
TTATACA50.045.035
TACACAT50.045.038
Produced by FastQC (version 0.11.2)