FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pa_85.351000000441d5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pa_85.351000000441d5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199473
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC10023650.250409829901784No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT3586417.979375654850532No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCG140587.047570347866629No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC105500.044.59478845
TTGTAAC250.002102944836.02
GCCGTCT950.033.15789445
ATGCCGT142250.033.0263644
AGTACTC352.8120805E-432.1428575
TAATATT300.005137184629.99999833
TATGCCG156700.029.98085443
GTATGCC157250.029.87599242
CGTATGC157600.029.86674941
CCCACGA158450.029.83433319
ATCTCGT157550.029.83338537
GCCCACG158350.029.82475518
TCTCGTA157450.029.82375538
CACGAGA158450.029.82013521
CCACGAG158550.029.81551620
ACGAGAC158400.029.8153422
TCGTATG157800.029.8146440
CGAGACA158300.029.80574623
AGCCCAC158500.029.7965317
TATCTCG157600.029.79536636
Produced by FastQC (version 0.11.2)