FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pa_83.35100000044192.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pa_83.35100000044192.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences236398
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC131475.561383768052183No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT58822.488176718923172No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCG16680.7055897258014027No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC4480.18951090956776284No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC4190.17724346229663535No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA3900.16497601502550782No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA3060.1294427194815523No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC3000.12690462694269833No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA2840.12013638017242109No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA2700.11421416424842849No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC2680.11336813340214384No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC2610.11040702544014755No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATAC2430.10279274782358563No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA2420.10236973240044331No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC14100.044.5183545
TAGGTAA250.002104410435.9976334
ATGCCGT19850.031.62260444
AGGTACT300.005130108430.0107254
CGCATAA300.005130108430.0107254
ACCCGCT453.4707613E-530.0107236
CTTTATA453.4707613E-530.0107232
CACTGTG300.00514073929.9980287
CCGCTGA453.4808076E-529.9980268
TATGCCG21950.028.59720843
GTATGCC22200.028.27516642
CGTATGC22250.028.21162641
CTCGTAT22250.028.21162639
TCGTATG22300.028.14837340
TATCTCG22250.027.90827636
TCTCGTA22650.027.81273838
ATCTCGT22550.027.73653437
ATAATAT22350.027.68274332
AATATCT22400.027.62094934
CATAATA22500.027.4981931