FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pa_82.35100000044178.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pa_82.35100000044178.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences309340
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC84332.7261265920993085No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT13150.42509859701299546No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6190.2001034460464214No Hit
GGATTATGACTGAACGCCTCTAAGTCAGAATCCGGGCTAGAAGCGACGCAT4950.16001810305812375No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTC4660.15064330510118318No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGC4310.13932889377384108No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCG4300.13900562487877416No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG4150.1341565914527704No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4120.13318678476756965No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3670.11863968448955842No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3580.11573026443395615No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA3370.10894161763755092No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTCT3340.10797181095235017No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA3220.10409258421154716No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAATAC207.015836E-445.012293
TGCCGTC9400.038.77464745
ATGCCGT9450.038.33140644
CGTATGC9950.036.8574441
CTGCGCG1100.036.8163269
TAACCTA1100.036.81632617
TATGCCG9900.036.5890743
TCGTATG10000.036.4481740
CTCGTAT10050.036.26683439
GTATGCC10050.036.26683442
GCATTAG250.002098578736.021481
ATAATAT10200.035.95407532
CGAGACA10100.035.86453223
ATCTCGT10200.035.73349837
TATCTCG10200.035.73349836
TCTCGTA10200.035.73349838
ACTGCGC1200.035.623218
TAATATC10300.035.60500733
CATAATA10300.035.60500731
ACATAAT10350.035.43300230
Produced by FastQC (version 0.11.2)