FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pa_81.3510000004416b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pa_81.3510000004416b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences244470
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC61302.5074651286456415No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC42271.7290465087740827No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC22850.934675011248824No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA20900.8549106229803248No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20870.8536834785454248No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG19040.7788276680165255No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC17490.7154252055466929No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGT16930.6925185094285597No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC16080.6577494171063935No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA15510.6344336728432937No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12250.5010839775841616No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11890.4863582443653618No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11790.4822677629156951No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA11700.47858632961099523No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGC11470.469178222276762No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11380.46549678897206204No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10470.42827340778009576No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATA10320.4221376856055958No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA10050.4110933856914959No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA9400.38450525626866283No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9060.3705976193397963No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT8830.3611895120055631No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8330.3407371047572299No Hit
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGG8060.32969280484313No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAAC7810.3194666012189635No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT7650.3129218308994969No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGT7620.31169468646459686No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA7620.31169468646459686No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGG7460.3051499161451303No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7300.29860514582566366No Hit
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7200.29451466437599705No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA7150.29246942365116374No Hit
CTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATAC6790.2777436904323639No Hit
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACC6600.2699717756779973No Hit
CAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACG6560.26833558309813066No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCA6400.26179081277866406No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCACT5820.23806602037059763No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA5580.22824886489139773No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTG5310.21720456497729781No Hit
ACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTT5220.21352313167259787No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA5050.2065693132081646No Hit
GCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTAC4670.19102548369943143No Hit
ACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGC4630.18938929111956476No Hit
GTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCA4600.1881621466846648No Hit
GAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA4540.18570785781486482No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA4500.18407166523499816No Hit
GCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTAC4490.1836626170900315No Hit
GTATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA4360.1783449912054649No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA4290.17548165419069825No Hit
GTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTAC4270.17466355790076493No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC4180.17098212459606496No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCT3990.16321020984169837No Hit
ATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCA3990.16321020984169837No Hit
CTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGA3870.1583016321020984No Hit
TACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGC3860.15789258395713177No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAA3590.14684828404303185No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA3490.14275780259336524No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA3330.13621303227389864No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG3230.132122550824232No Hit
TATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAG3180.13007731009939869No Hit
GTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3180.13007731009939869No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA3080.12598682864973207No Hit
GTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGC3000.12271444348999877No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT2860.1169877694604655No Hit
GTACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA2810.11494252873563218No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT2740.11207919172086554No Hit
GGTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAA2690.11003395099603223No Hit
ATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA2670.10921585470609892No Hit
GGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGC2670.10921585470609892No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT2670.10921585470609892No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA2650.10839775841616557No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCG2620.1071706139812656No Hit
CTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCC2620.1071706139812656No Hit
TATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA2600.10635251769133226No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA2550.10430727696649897No Hit
CAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGG2550.10430727696649897No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAG2490.10185298809669899No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC2480.10144393995173231No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATAC2470.10103489180676566No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC7100.042.14635545
ATGCCGT7600.039.07752644
GGGCGCA356.2296804E-638.57002618
ATAATAT8000.037.9673732
CATAATA8000.037.9673731
TATCTCG7950.037.9231536
TCTCGTA7950.037.9231538
CACGAGA8100.037.77640521
AACATAA8050.037.7315529
AGACAAC8050.037.7315525
ACATAAT8050.037.7315530
ATCTCGT8000.037.68612737
ATATCTC8000.037.68612735
TATGCCG7900.037.5935743
GTATGCC7900.037.5935742
CCACGAG8150.037.54464720
ACAACAT8100.037.4986427
CAACATA8100.037.4986428
GAGACAA8100.037.4986424
CGAGACA8100.037.4986423
Produced by FastQC (version 0.11.2)