FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pa_8.35100000043831.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pa_8.35100000043831.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1252
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC221.7571884984025559RNA PCR Primer, Index 27 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGT80.6389776357827476No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC40.3194888178913738No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCG30.23961661341853036No Hit
AATTCAATGATGATTACATTGGATTCCGTTCTATGATTCCATTCAATTCCG30.23961661341853036No Hit
CGCCCAGGCGCAGCAGACGGCTGCCCCGGCCGCTGCCACTACCAAGGCGGC20.1597444089456869No Hit
ACACTGGCTTATCTGAATTGATGTCTAAGGAAATGGTAATGTTTATTAACT20.1597444089456869No Hit
AGCCGATACCGACCGACAGCGCGCTGACGATCCACGCCAGGTTTTCCCATT20.1597444089456869No Hit
GGATACCGTGCTGTTCGGGCCCTTCAGTCCAAATTCCATGGACAAATGCGC20.1597444089456869No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCT20.1597444089456869No Hit
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGG20.1597444089456869No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT20.1597444089456869No Hit
TAAATAAACATAGCCCTCATATTTGTAACAGAACCAGTATCCCAATTTCCG20.1597444089456869No Hit
CTTAAAAAGAGCTAAGTAACCAACAAATTAACAGCATAATCACAATAAACT20.1597444089456869No Hit
GTACAGGCTAAAGCAGAATTTGAGGGTGAAATAAATAAGAAACAGAAATGA20.1597444089456869No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAACTGTCTCTTATACA20.1597444089456869No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1597444089456869No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA20.1597444089456869No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)