FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pa_79.35100000044128.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pa_79.35100000044128.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78014
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC21172.713615504909375No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGC3050.39095546953110977No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT2620.3358371574332812No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTCT2000.2563642423154819No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG1900.24354603019970775No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT1690.21662778475658218No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCG1670.21406414233342735No Hit
GCGCTTAAGCGCGCGACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTC1580.20252775142923066No Hit
GTGCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGATTAA1570.20124593021765325No Hit
CTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTTG1420.18201861204399208No Hit
GGGTAAAGCCAGAGGAAACTCTGGTGGAAGCCCGCAGCGATACTGACGTGC1410.18073679083241467No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCC1370.17560950598610506No Hit
GTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCGAT1350.17304586356295024No Hit
ACGTTAGGGAGTCCGGAGACGTCGGCGGGGGCCTCGGGAAGAGTTATCTTT1330.17048222113979541No Hit
CTTGTGGAGACGTCGCTGCCGTGATCGTGGTCTGCAGCACGCGCCTAACGG1320.169200399928218No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA1300.16663675750506318No Hit
GGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGAATCGAGAGCTC1290.16535493629348577No Hit
GTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCTT1290.16535493629348577No Hit
TGCCCGGACCCTGTCGCACCACGAGGCGCTGTCTACGAGTCGGGTTGTTTG1280.16407311508190836No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGCT1260.16150947265875357No Hit
GCCTAAGCCCGGGCTTTTGATACGCTTGTGGAGACGTCGCTGCCGTGATCG1200.1538185453892891No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGGA1170.14997308175455687No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA1170.14997308175455687No Hit
GGCTTAGGCCGCCACCGTAATCCGCGTCGGTCCACGCCCCGAATCGATCGG1160.14869126054297946No Hit
ACTTAGGCCCGGTCCGTCGCTGAGGACGCTTCTCCAGACTACAATTCGAAC1140.14612761811982464No Hit
GGCCTAAGTTCCCTGGAAAGGGGCGCCAGAGAGGGTGAGAGCCCCGTCGTG1110.14228215448509243No Hit
GTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGC1100.14100033327351502No Hit
ACCGTAATCCGCGTCGGTCCACGCCCCGAATCGATCGGCGGACCGGATTGC1100.14100033327351502No Hit
TGCCTACATTGTTCCATCGACCAGAGGCTGTTCACCTTGGAGACCTGATGC1050.13459122721562797No Hit
CCGTTAGGCGCGTGCTGCAGACCACGATCACGGCAGCGACGTCTCCACAAG1040.13330940600405056No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTC1040.13330940600405056No Hit
ATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCTCGG990.12690029994616353No Hit
ACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCGATGGAAC990.12690029994616353No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATG990.12690029994616353No Hit
GTCAAAGAGTGCTTGAAATTGTCGGGAGGGAAGCGGATGGGGGCCGGCGAT980.12561847873458612No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCGGA940.12049119388827646No Hit
CTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTA930.11920937267669905No Hit
ATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGAAAAGGAT920.11792755146512164No Hit
GGATTGCTCCGTTCCGCATCCGACCAGGACGCATCGCCGGCCCCCATCCGC910.11664573025354423No Hit
TAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGACGTCTT870.11151844540723461No Hit
AACAAGTACCGCGAGGTAAAGATGAAAAGGACTTTGAAAAGAGAGTCAAAG860.1102366241956572No Hit
GGTTCCATGTGAACGGCACTTGCACATGGGTTAGTCGATCCTAAGAGTCGG850.1089548029840798No Hit
CCCTGGAAACGGCTCAGCCGGAGGTAGGGTCCAGCGGCTGGAAGAGCACCG840.10767298177250238No Hit
GGGGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTTAGTCGATCCTAA840.10767298177250238No Hit
GGGGAAACCCGTCTGATAGCGCTTAAGCGCGAACTTCGAAAGGGGATCCGG830.10639116056092497No Hit
GGGTTACGGTGCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGG820.10510933934934756No Hit
GGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCG820.10510933934934756No Hit
CGCCTAACGGCGTGCCTCGGCATCAGCGTGCTCCGGGCGTCGGCCTGTGGG820.10510933934934756No Hit
GGCTTTACCCCGCTCAGGCATAGTTCACCATCTTTCGGGTCCCGACAGGCA820.10510933934934756No Hit
AGCCCGGGCGGAGCGGCCGTCGGTGCAGATCTTGGTGGTAGTAGCAAATAT800.10254569692619274No Hit
TTACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTT800.10254569692619274No Hit
GGATTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAA800.10254569692619274No Hit
CATGTCAGACTCCTTGGTCCGTGTTTCAAGACGGGTCGAATGGGGAGCCCA800.10254569692619274No Hit
GGCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTAAAGA790.10126387571461533No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCTAG301.1301952E-437.50000437
CGGAGGT250.00209511536.019
GGCTCAG250.00209511536.011
GGAGGTA250.00209511536.020
AAACGGC250.00209511536.07
GCTCAGC250.00209511536.012
ATGCCGT2850.035.52631844
GTATGCC2900.034.9137942
TGCCGTC2900.034.9137945
TATGCCG2900.034.9137943
CACGAGA2900.034.9137921
ACGAGAC2900.034.9137922
CGTATGC2900.034.9137941
TCGTATG2900.034.9137940
CTCGTAT2900.034.9137939
ACTTGCT2850.034.73684327
CTTGCTG2850.034.73684328
CGAGACT2850.034.73684323
GACTTGC2850.034.73684326
GCCCACG2950.034.32203318
Produced by FastQC (version 0.11.2)