FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pa_77.351000000440e3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pa_77.351000000440e3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences313355
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC9175729.282124108439312No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT4085013.036332594022754No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCG56461.8017903017344543No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAACA11270.3596559812353401No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC10640.3395509884954764No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA9520.3038087791801631No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC8240.26296053996266217No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGAG7080.22594182317180195No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGG6980.22275055448293468No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA6300.20104992739863733No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA5440.1736050166743789No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGAT5230.16690335242775767No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC5150.16435033747666383No Hit
GTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGAGCCCACGA5020.16020168818113642No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT4810.15350002393451517No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC4630.1477557402945541No Hit
AAACATCCTTGGCAAATGCTTTCGCAGTTGTTCGTCTTTCATAAATCCAAG4470.14264971039236649No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGATG4270.13626717301463198No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA4100.13084201624355762No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT4060.12956550876801073No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCG4020.12828900129246382No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTC3990.12733162068580364No Hit
CCCAACTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGCA3910.12477860573470984No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA3670.1171195608814284No Hit
AAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCACG3470.1107370235036939No Hit
CTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCC3280.1046736129948461No Hit
TGTTCGTCTTTCATAAATCCAAGAATTTCACCTCTGACTATGAAATACGAA3280.1046736129948461No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGAG3240.10339710551929919No Hit
GGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGAGCCCACG3190.10180147117486557No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT302.1610103E-644.9982545
AGACTTT207.026774E-444.99824525
TTACTGC207.026774E-444.99824527
GACTTTC207.026774E-444.99824526
TACTGCA207.026774E-444.99824528
ACGCTTA207.026774E-444.99824514
TGTAACG207.026774E-444.99824510
GTAACGC207.026774E-444.99824511
TGCCGTC92800.044.29514345
TACCCGC552.728484E-936.82855
ACCCGCT552.732122E-936.822626
CCCGCTG552.737579E-936.8167467
GGACAAA250.00210188236.010091
ACTGCAC250.00210517835.99859629
GCTGCAG250.00210517835.99859638
AAAGTGC250.00210517835.99859633
GACTTAC250.00210517835.99859626
GTGCTGC250.00210517835.99859636
CTGCAGA250.00210517835.99859639
GCCGTCT1250.034.19866645
Produced by FastQC (version 0.11.2)