FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pa_75.351000000440a0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pa_75.351000000440a0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246755
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC187667.605114384713582No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT53642.173816133411684No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCG9170.3716236753054649No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC8870.3594658669530506No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC6840.2771980304350469No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA6310.2557192356791149No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTCTGTCTCTTATACACATCTCCGA6200.25126137261656295No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA4470.18115134445097364No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA3210.13008854937083342No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA2990.12117282324572956No Hit
AAAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG2920.11833600129683289No Hit
GGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGAGC2900.11752548074000528No Hit
AAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAG2870.11630969990476384No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATAC2820.11428339851269477No Hit
AAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGC2660.10779923405807379No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG207.016278E-445.0081063
AGCGTCG207.0232886E-444.99898521
GCGTCGT207.0232886E-444.99898522
ACCTTAG207.0232886E-444.99898512
TGCCGTC19450.044.18923645
GCCGTCT301.1374764E-437.49915745
ATGGGTC250.002102050236.0064855
TGGGTCC250.002104142735.9991876
GGTCCTC250.002104142735.9991878
CCGTATA250.002104142735.99918739
GCCGTAT250.002104142735.99918738
CCTCTAG250.002104142735.99918711
ATGCCGT25700.033.44282544
TATGCCG26400.032.6413143
TCTCGTA26450.032.4945438
AATCTCG26400.032.47086336
ATCTCGT26500.032.4332337
CTCGTAT26500.032.4332339
GTATGCC26500.032.4332342
CGTATGC26600.032.39588541
Produced by FastQC (version 0.11.2)