FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pa_73.3510000004406c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pa_73.3510000004406c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences495202
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC24890.50262317195811No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG6900.13933707860630612No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGC5900.1191432990981458No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC5370.10844059595882084No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTC5030.10157471092604634No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC2900.038.01638442
TATGCCG3050.036.1467343
ATGCCGT3050.036.1467344
TAGGGGA250.002105141536.0028274
CGTATGC3100.035.56371741
TCGTATG3050.035.4090440
CTCGTAT3000.035.24920739
GCCCACG3350.032.9097118
CCCACGA3300.032.7265419
CGAGACT3400.032.42574323
TCTCGTA3400.031.10224338
CGGGATA300.005142487630.0023564
CACGAGA3750.028.79935321
GACTTGC3850.028.6357226
ACTTGCT3900.028.26859727
ATCTCGT3850.027.46691537
TGCCGTC4150.027.10782445
CTCCGAG4200.026.78511412
GAGACTT4150.026.56566824
ACGAGAC4150.026.56566822
Produced by FastQC (version 0.11.2)