FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pa_72.35100000044043.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pa_72.35100000044043.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176366
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC2261412.822199290112607No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT98465.582708685347516No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCG13270.7524125965322115No Hit
ACAAACATAAGTCTCAGCCCTCAAAAATTTCCTGTTATGTCTTTGATTCTT1790.10149348513885897No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGCG207.0183404E-444.99830220
CGTAGGA207.0183404E-444.99830222
TGCGTCC207.0183404E-444.99830212
TGCCGTC25200.043.83762745
TTATGTC301.1362601E-437.4985835
TAGCGAG250.00210267535.99864222
ATCGTAG250.00210267535.99864238
AGCGAGA250.00210267535.99864223
ATGCGAG250.00210267535.99864211
ACTGGAT401.5535172E-533.74872613
GTCTCAG352.8113913E-432.14164411
ATGCCGT35700.030.81816344
AGTAGGT300.00512941630.0073743
GAGGTCC453.475182E-529.99886740
CGTAGAG300.00513653729.99886540
TGCGAGA300.00513653729.99886512
ATCTCGT37400.029.59781337
TATCTCG37350.029.57719436
CGCTATA37500.029.51888331
TCTCGTA37350.029.51695638
Produced by FastQC (version 0.11.2)