FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pa_70.35100000043ff4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pa_70.35100000043ff4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences302288
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC210496.963227121155984No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT94703.132774043296459No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCG9920.3281638702164823No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTAG206.982748E-445.0551571
GCCAGTT207.028465E-444.99553327
TGCCGTC22150.044.081445
TAATACA301.1341681E-437.5211034
CGCTAGA250.002098851836.020262
TCCGCTG250.002105683635.99642633
GGTGTGT250.002105683635.99642624
CATCGTG250.002105683635.9964269
TTAGTGT250.002105683635.9964269
GGGCAGA250.002105683635.99642620
GCCGTCT501.9767685E-631.49687245
ATGCCGT32000.030.51259444
GAACTAT300.005110615430.0367741
ACAGGTC300.005143824529.997028
CGGGCAG300.005143824529.9970217
AGGGCAG300.005143824529.9970219
TATGCCG33150.029.52195543
GTATGCC33300.029.45653542
CGTATGC33350.029.34491241
TCGTATG33350.029.34491240
Produced by FastQC (version 0.11.2)