FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pa_69.35100000043fda.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pa_69.35100000043fda.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166261
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC11448368.85739890894436No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT1973511.869891315461834No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCG69214.162732089906833No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCTGTC2240.1347279277762073No Hit
CCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT1750.10525619357516194No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACATCC207.015869E-445.0000047
TGCAGTC207.015869E-445.00000445
TTTATAC351.2035525E-745.03
TGCCGTC118200.044.60025445
ATGCCGT138150.038.1596144
CCATATC301.13572685E-437.49999633
GCCATAT301.13572685E-437.49999632
CATATCT301.13572685E-437.49999634
TATATCT145200.036.1828534
ATATCTC145550.036.17313835
GCCCACG146600.036.1596218
CCCACGA146600.036.1596219
TATGCCG145800.036.15740643
AGCCCAC146550.036.15660517
CCACGAG146750.036.15332420
CTATATC145450.036.15159633
ACGCTAT145450.036.15159630
TATCTCG145700.036.1513436
CACGAGA146650.036.1472921
ATCTCGT145850.036.1450137
Produced by FastQC (version 0.11.2)