FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pa_68.35100000043fb0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pa_68.35100000043fb0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104817
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC33803.224667754276501No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT8420.8033048074262763No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT2590.24709732199929402No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC2550.24328114714216204No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA2140.20416535485655954No Hit
TTGTAGTACTTGGCTGATTCTTTCTCATAACGTGTGAGGTTATTTTTGAAA2130.20321131114227653No Hit
GTTACAAAAACAGATTCGGACTCTATTACGTCGATTTCAAAAATAACCTCA1970.1879466117137487No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA1610.15360103799956112No Hit
CCGTTACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATG1550.1478767757138632No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA1370.1307039888567694No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC1280.12211759542822252No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC1240.11830142057109055No Hit
TTGTAATACTTGGCTGATTCTTTCTCATAACGTGTGAGGTTATTTTTGAAA1190.11353120199967563No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG1170.11162311457110966No Hit
GTTATAATAACAGATTCGGACTCTATTACGTCGATTTCAAAAATAACCTCA1160.11066907085682666No Hit
CTATTACGTCGATTTCAAAAATAACCTCACACGTTATGAGAAAGAATCAGC1140.10876098342826068No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC1070.10208267742827977No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4100.042.2529645
ATGCCGT4850.036.18288444
ATAATAC250.0020942536.014513
CGACGCT5050.034.74989728
CGCTATA5050.034.74989731
GACGCTA5050.034.74989729
CGTATGC5100.034.4092141
TATGCCG5100.034.4092143
GTATGCC5100.034.4092142
GACGACG5100.034.4092126
CTCGTAT5150.034.0751439
CTATATC5150.034.0751433
ACGCTAT5150.034.0751430
CGAGACG5150.034.0751423
ACGACGC5150.034.0751427
ATCTCGT5200.033.74749437
GCTATAT5200.033.74749432
TATATCT5200.033.74749434
CACGAGA5200.033.74749421
AGACGAC5200.033.74749425
Produced by FastQC (version 0.11.2)