FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pa_66.35100000043f7d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pa_66.35100000043f7d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119237
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC32842.7541786525994447No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT6340.5317141491315615No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT2000.1677331700730478No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA1590.133347870208073No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA1200.10063990204382868No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCAGG206.995288E-445.016371
TAGAACG207.009773E-444.99748223
TGCCGTC3950.039.8711945
ATGCCGT4350.036.20487244
TTTATAC250.002095810136.0130923
CGCTATA4600.035.21542431
TATGCCG4500.034.99804343
GTATGCC4500.034.99804342
CGTATGC4650.034.8367641
TCGTATG4650.034.8367640
GACGCTA4650.034.8367629
CTCGTAT4700.034.46615639
CGACGCT4700.034.46615628
CTATATC4700.034.46615633
CGAGACG4700.034.46615623
ACGACGC4700.034.46615627
GCTATAT4750.034.10335532
CCCACGA4750.034.10335519
TATCTCG4750.034.10335536
AGACGAC4800.033.7481125
Produced by FastQC (version 0.11.2)