FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pa_64.35100000043f3a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pa_64.35100000043f3a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162046
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC2664616.443479012132357No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGT1958112.083605889685645No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCG32391.9988151512533476No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCA206.997089E-445.023784
ACTACTC206.997089E-445.023783
TGCCGTC28900.043.98396745
CTAGACT301.1320695E-437.5198144
GCCGTCT601.5279511E-1037.49665545
TCCTGCT250.002096335236.0190242
GTAGGTG250.002096335236.0190245
CTAAGAC250.002096335236.0190243
TGTAGGA250.002096335236.0190242
GGACAGT250.002096335236.0190246
ACTAGAC458.603056E-735.0184943
AGACTGA401.546728E-533.7678386
CTGTCTG352.811234E-432.13998819
ACTGATC352.811234E-432.1399888
TGTCTGA352.811234E-432.13998820
TCATCCG352.811234E-432.13998834
CATCCGT352.811234E-432.13998835
CTAGGAG300.005121113330.0158524
TAGACTG453.4600453E-530.015855
GATAAGT300.00513660729.99732426
Produced by FastQC (version 0.11.2)