FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pa_63.35100000043f10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pa_63.35100000043f10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116277
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC1205610.368344556533106No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGT17451.5007267129354902No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCG4180.3594863988578997No Hit
ATCGAAAAGAATCGAATCGAAAAGAATTGAATCGAATCGAAAAGAAACGAA2080.17888318412067736No Hit
AATTAAGTGTCCTCCATGATCTACAGGATTAGTAACTATTCCGTCTTTTAT1770.15222270956423023No Hit
GAGTATTAAGTGCTATCAATACCGCTTTATCAAAGAACAACATCAATATAG1650.14190252586496038No Hit
GAAGAAATTCATATGAAGAGACAGATTTTTTAGATCCATTTTGGCAAAAGA1540.13244235747396305No Hit
ATCTTTGAAGTATGACCATAAACAGTTCATTTCATTGTTCCCTGCCTCACT1520.13072232685741805No Hit
ATGATATTCGTATCTTGCGTGATATTGAGCAATATTACTCCACACAAATTG1420.12212217377469319No Hit
GTGTATTTCATCTGGTGTAAAATCTCAGCTGGTGTAAAACGCAGTGTATTT1410.12126215846642072No Hit
GAATGCAATGGAATGGAATGGAATAACATGCAATCACCTTTGGTGGAATGG1300.11180199007542334No Hit
ATTATTGCTTTCCGAAAAATTGGAGGTATGCATAGATACTTCATCTCCACA1290.11094197476715087No Hit
ACAGACGGAAAGGAAAGAAACACGAAAAAGAGAGAAAGAATGAGGAAAGAA1250.10750191353406091No Hit
CTATGTGGATGGAAAAATAGAAGGTGCTGTTATTTGGAATCATCCAAATGG1230.10578188291751595No Hit
ATAACAAATGTGCATAAGGCATAGATACTGTAAACGGCTTGTATTCCTTTA1190.10234182168442599No Hit
AACAAGAGTGGAATGGAATGGAATGGAATGAAATGGAATGGAATGGAATGG1180.10148180637615349No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG253.8693528E-544.99957322
TCGCACA253.8693528E-544.9995739
TAAACGG302.1487867E-644.9995731
CCAGAGT207.0074515E-444.9995713
AAACGGC302.1487867E-644.9995732
GAGGTCA207.0074515E-444.999576
CTTGCGT207.0074515E-444.9995714
CGCAGTG207.0074515E-444.9995740
CAGAGAT207.0074515E-444.9995739
GAGATCT207.0074515E-444.9995741
GTAAACG302.1487867E-644.9995730
TGCCGTC14300.043.11147345
GGCTTGT356.20038E-638.57105636
AACGGCT356.20038E-638.57105633
AAGGCAT356.20038E-638.57105616
AATGTGC356.20038E-638.5710567
CGGGTTC301.1336854E-437.4996421
ACGCAGT301.1336854E-437.4996439
CTTCAAG301.1336854E-437.4996442
AACGGGT301.1336854E-437.4996419
Produced by FastQC (version 0.11.2)