FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pa_62.35100000043ef5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pa_62.35100000043ef5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378787
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC19564551.650399828927604No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGT6089816.077109298893575No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCG199745.273148233703902No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACA5630.14863234482704002No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATACA5490.14493633625230012No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATAC4650.12276028480386074No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATAC4580.1209122805164908No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACATC207.0234324E-445.0050166
GTATGCT502.1827873E-1144.99907342
TATGTCG207.028003E-444.99907344
TGCCGTC201250.044.7419445
TATGCTG501.0768417E-940.49916543
TCTACGA301.13861504E-437.49922611
CTACGAG301.13861504E-437.49922612
TGCGTAA301.13861504E-437.49922619
GCCGTCT1050.036.42782645
ATATACA250.002104177436.0040134
TATACAT250.002104177436.0040135
GCCCCAC250.002105541535.9992618
CATGGCG250.002105541535.9992629
ATCTCAT250.002105541535.9992637
GCCCACA250.002105541535.9992618
CATATGC250.002105541535.9992641
AGCCCCA250.002105541535.9992617
AGCTTGC250.002105541535.9992615
GACCATG250.002105541535.9992626
GAGACTA250.002105541535.9992624