FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pa_61.35100000043edb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pa_61.35100000043edb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138275
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC7310752.87072862050262No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGT2208915.974688121497016No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCG56514.086783583438799No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGTG207.013137E-444.9981918
GGGGTGG207.013137E-444.9981919
TGCCGTC75950.044.4945945
GCCGTCT850.037.0573345
GTATGCT250.002101130835.99855442
ACGGGGT250.002101130835.99855417
GGGTGGT250.002101130835.99855420
ATGCCGT99150.034.06065844
GGTGGTA352.808306E-432.14156721
AATCTCG106000.031.92324636
TCTCGTA106400.031.90896438
TATGCCG105850.031.90471843
CGTATGC106500.031.90012641
TCGTATG106500.031.90012640
AGCCCAC106800.031.89478517
CTCGTAT106400.031.88781739
CTCCGAG107000.031.87722612
CCCACGA106800.031.87371819
ATCTCGT106450.031.87283937
GTATGCC106050.031.86576342
Produced by FastQC (version 0.11.2)