FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pa_60.35100000043eb1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pa_60.35100000043eb1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225530
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC32181.4268611714627766No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGT6840.3032855939342881No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3950.041.5785945
ATGCCGT4600.035.70335444
TATGCCG4750.034.57588243
CGTATGC4800.034.21571741
CAAGGCG4750.034.10223829
GGCGAAT4750.034.10223832
TCGTATG4850.033.86297640
GTATGCC4900.033.51743742
AGGCGAA4850.033.399131
CGAGACC4850.033.399123
CTCGTAT4950.033.17887539
AAGGCGA4950.032.72437330
ATCTCGT5050.032.5218737
CCCACGA5050.032.5218719
TCTCGTA5050.032.5218738
CCACGAG5050.032.5218720
AATCTCG5100.032.20302636
GCGAATC5050.032.07636333
CCAAGGC5050.032.07636328
ACGAGAC5150.031.89037722
Produced by FastQC (version 0.11.2)