FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pa_6.351000000437fc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pa_6.351000000437fc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182604
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC27961.5311822304002103RNA PCR Primer, Index 27 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGT4150.2272677487897308No Hit
ATATAATTCTCTTCTTCACATACAGCAGTTTAGATTCACAATACAACAAGC3070.1681233707914394No Hit
ATATAATTCTCTTCTTCACATACAGCGGTTTAGATTCACAATACAACAAGC2930.16045650697684605No Hit
ATATATGATTGACCATAACACGATGTGATTACAATCAATTACAAAGCCAAA2730.1495038443845699No Hit
CTTTACATCCTCATATTCATCTGTTAATTTGGCTTTGTAATTGATTGTAAT2470.13526538301461083No Hit
GTATAGGTAAGCATTGCATGCTTATAAACCGATGTATGCAAGTAATAACTC2040.11171715844121707No Hit
CTTATAAACCGATGTATGCAAGTAATAACTCTGTAAAAAAACGTTACAGAT1830.10021686271932707No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATATA207.001917E-445.0205462
TATGGGA253.8799757E-544.9958910
TGCCGTC2850.041.83828745
CAGCGGT403.439418E-739.37140723
TACAGCG403.439418E-739.37140721
TAGGATA356.199991E-638.5890434
AAGCGCC356.2233667E-638.56790519
GTATTAT301.12763926E-437.547991
TATACTA301.133114E-437.5171245
CTATGGG301.136776E-437.496589
ACTATGG301.136776E-437.496588
GTATGCC3400.035.07032842
GCGCCAT458.6529326E-734.99680721
CGTATGC3450.034.56206541
CCCACGA3450.034.56206519
ATGCCGT3450.034.56206544
TATGCCG3500.034.06831743
GAATTGC3500.034.06831729
GGTCGCG401.5543874E-533.7469187
GCTATCT3550.033.58848634