Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l02n01_bir_0113_pa_6.351000000437fc.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 182604 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 44 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC | 2796 | 1.5311822304002103 | RNA PCR Primer, Index 27 (95% over 21bp) |
TCTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGT | 415 | 0.2272677487897308 | No Hit |
ATATAATTCTCTTCTTCACATACAGCAGTTTAGATTCACAATACAACAAGC | 307 | 0.1681233707914394 | No Hit |
ATATAATTCTCTTCTTCACATACAGCGGTTTAGATTCACAATACAACAAGC | 293 | 0.16045650697684605 | No Hit |
ATATATGATTGACCATAACACGATGTGATTACAATCAATTACAAAGCCAAA | 273 | 0.1495038443845699 | No Hit |
CTTTACATCCTCATATTCATCTGTTAATTTGGCTTTGTAATTGATTGTAAT | 247 | 0.13526538301461083 | No Hit |
GTATAGGTAAGCATTGCATGCTTATAAACCGATGTATGCAAGTAATAACTC | 204 | 0.11171715844121707 | No Hit |
CTTATAAACCGATGTATGCAAGTAATAACTCTGTAAAAAAACGTTACAGAT | 183 | 0.10021686271932707 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TAATATA | 20 | 7.001917E-4 | 45.020546 | 2 |
TATGGGA | 25 | 3.8799757E-5 | 44.99589 | 10 |
TGCCGTC | 285 | 0.0 | 41.838287 | 45 |
CAGCGGT | 40 | 3.439418E-7 | 39.371407 | 23 |
TACAGCG | 40 | 3.439418E-7 | 39.371407 | 21 |
TAGGATA | 35 | 6.199991E-6 | 38.589043 | 4 |
AAGCGCC | 35 | 6.2233667E-6 | 38.567905 | 19 |
GTATTAT | 30 | 1.12763926E-4 | 37.54799 | 1 |
TATACTA | 30 | 1.133114E-4 | 37.517124 | 5 |
CTATGGG | 30 | 1.136776E-4 | 37.49658 | 9 |
ACTATGG | 30 | 1.136776E-4 | 37.49658 | 8 |
GTATGCC | 340 | 0.0 | 35.070328 | 42 |
GCGCCAT | 45 | 8.6529326E-7 | 34.996807 | 21 |
CGTATGC | 345 | 0.0 | 34.562065 | 41 |
CCCACGA | 345 | 0.0 | 34.562065 | 19 |
ATGCCGT | 345 | 0.0 | 34.562065 | 44 |
TATGCCG | 350 | 0.0 | 34.068317 | 43 |
GAATTGC | 350 | 0.0 | 34.068317 | 29 |
GGTCGCG | 40 | 1.5543874E-5 | 33.746918 | 7 |
GCTATCT | 355 | 0.0 | 33.588486 | 34 |