FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pa_59.35100000043e98.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pa_59.35100000043e98.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215233
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC27041.256312925991832No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGT10550.4901664707549493No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC2800.044.1950645
GTCTCAG352.8133264E-432.1418625
CGGGGTG352.8133264E-432.1418618
ATGCCGT3800.031.97269644
CGTTACT809.822543E-1130.9437312
CCGTTAC751.6843842E-930.006041
CGGGTAT300.00513885229.99907317
CGTATGC4150.028.73404941
TATGCCG4250.028.5873543
CAAGGCG4250.028.5873529
GGCGAAT4300.028.2549432
GTACCGC406.1424944E-428.1306656
ACCGCGA406.1508524E-428.124138
TACCGCG406.1508524E-428.124137
GCGAGGT406.1508524E-428.1241311
GCGAATC4350.027.93016833
AGGCGAA4350.027.93016831
CGAGACC4450.027.80812623
ACGAGAC4450.027.80812622
CCAAGGC4400.027.61278228
Produced by FastQC (version 0.11.2)