FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pa_58.35100000043e7e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pa_58.35100000043e7e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30155
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC4011.329796053722434No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC890.2951417675344056No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGT570.1890233792074283No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA480.15917758249046593No Hit
GGAGGAGGAGAAATTGAAGTGCTTTTGATAAGTGCACAGAAGAAAGGGAAA400.1326479854087216No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC330.10943458796219532No Hit
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGG320.10611838832697729No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG310.10280218869175924No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC700.045.045
GCCCACG750.042.00000418
TATGCCG750.042.00000443
CACGAGA750.042.00000421
CCCACGA750.042.00000419
ACGAGAC750.042.00000422
TCCGAGC750.042.00000413
GCGAATC700.041.78571333
GGCGAAT700.041.78571332
TCTCGTA700.041.78571338
CAAGGCG700.041.78571329
AGCCCAC800.039.37517
GTATGCC800.039.37542
ATGCCGT800.039.37544
CGTATGC800.039.37541
CCGAGCC800.039.37514
CTCCGAG800.039.37512
TCGTATG750.039.00000440
CGAGACC750.039.00000423
AGACCAA750.039.00000425