Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l02n01_bir_0113_pa_57.35100000043e55.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 35833 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 46 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC | 7843 | 21.887645466469454 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGT | 927 | 2.587000809309854 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCG | 240 | 0.6697736723132308 | No Hit |
ATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTCTTC | 128 | 0.3572126252337231 | RNA PCR Primer, Index 46 (95% over 22bp) |
TTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTCT | 49 | 0.1367454580972846 | No Hit |
TATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTCTT | 48 | 0.13395473446264616 | RNA PCR Primer, Index 46 (95% over 21bp) |
ATCGTATAGTGGTAGAAAAGTGTATGTGGTAGGGGTTATCAAAAAACAAGG | 45 | 0.12558256355873076 | No Hit |
AATATAGGTTTCCTCAACTGTAATGGGTAATATGCCCGATTCAAAGTATCC | 42 | 0.1172103926548154 | No Hit |
GAAGAGAATCGATTAGAATCGAAATGAAAAGAATCGTATTGAATCGAATCG | 42 | 0.1172103926548154 | No Hit |
GATATACATCGTGCAATGAACCAAGAAGAAAGACTCAGTAGATACCAACTG | 40 | 0.11162894538553846 | No Hit |
TTGTGTATCCTTTTGCTTGTTTTTTGTTGTGATAGTCTTTGAGTTACTCAA | 40 | 0.11162894538553846 | No Hit |
AAAATGAATTCTCAACCAGGTTGATGTTATTGCTGCTGAAGGATTTATTGT | 39 | 0.10883822175090001 | No Hit |
TCTTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCG | 36 | 0.10046605084698462 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
AGGATAC | 20 | 6.941998E-4 | 45.0 | 3 |
CACAAGC | 20 | 6.941998E-4 | 45.0 | 29 |
CAAAGTC | 20 | 6.941998E-4 | 45.0 | 9 |
CAGGATA | 20 | 6.941998E-4 | 45.0 | 2 |
TCACAAG | 20 | 6.941998E-4 | 45.0 | 28 |
AAGCATT | 25 | 3.815345E-5 | 44.999996 | 32 |
TGCCGTC | 965 | 0.0 | 41.96891 | 45 |
ATGCCGT | 1060 | 0.0 | 38.207546 | 44 |
AATCTCG | 1085 | 0.0 | 37.53456 | 36 |
ATCTCGT | 1085 | 0.0 | 37.53456 | 37 |
TCTCGTA | 1085 | 0.0 | 37.53456 | 38 |
CGTATGC | 1085 | 0.0 | 37.53456 | 41 |
GTATGCC | 1085 | 0.0 | 37.32719 | 42 |
GAATCTC | 1080 | 0.0 | 37.291668 | 35 |
CGAATCT | 1075 | 0.0 | 37.255814 | 34 |
TCGTATG | 1095 | 0.0 | 37.191784 | 40 |
CTCGTAT | 1095 | 0.0 | 37.191784 | 39 |
TATGCCG | 1090 | 0.0 | 37.15596 | 43 |
GAGACCA | 1080 | 0.0 | 37.083336 | 24 |
ATCTCCG | 1100 | 0.0 | 37.022728 | 10 |