FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pa_57.35100000043e55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pa_57.35100000043e55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35833
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC784321.887645466469454No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGT9272.587000809309854No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCG2400.6697736723132308No Hit
ATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTCTTC1280.3572126252337231RNA PCR Primer, Index 46 (95% over 22bp)
TTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTCT490.1367454580972846No Hit
TATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTCTT480.13395473446264616RNA PCR Primer, Index 46 (95% over 21bp)
ATCGTATAGTGGTAGAAAAGTGTATGTGGTAGGGGTTATCAAAAAACAAGG450.12558256355873076No Hit
AATATAGGTTTCCTCAACTGTAATGGGTAATATGCCCGATTCAAAGTATCC420.1172103926548154No Hit
GAAGAGAATCGATTAGAATCGAAATGAAAAGAATCGTATTGAATCGAATCG420.1172103926548154No Hit
GATATACATCGTGCAATGAACCAAGAAGAAAGACTCAGTAGATACCAACTG400.11162894538553846No Hit
TTGTGTATCCTTTTGCTTGTTTTTTGTTGTGATAGTCTTTGAGTTACTCAA400.11162894538553846No Hit
AAAATGAATTCTCAACCAGGTTGATGTTATTGCTGCTGAAGGATTTATTGT390.10883822175090001No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCG360.10046605084698462No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGATAC206.941998E-445.03
CACAAGC206.941998E-445.029
CAAAGTC206.941998E-445.09
CAGGATA206.941998E-445.02
TCACAAG206.941998E-445.028
AAGCATT253.815345E-544.99999632
TGCCGTC9650.041.9689145
ATGCCGT10600.038.20754644
AATCTCG10850.037.5345636
ATCTCGT10850.037.5345637
TCTCGTA10850.037.5345638
CGTATGC10850.037.5345641
GTATGCC10850.037.3271942
GAATCTC10800.037.29166835
CGAATCT10750.037.25581434
TCGTATG10950.037.19178440
CTCGTAT10950.037.19178439
TATGCCG10900.037.1559643
GAGACCA10800.037.08333624
ATCTCCG11000.037.02272810