FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pa_55.35100000043e11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pa_55.35100000043e11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150321
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC2332415.516128817663532No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT119517.950319649283866No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCG19091.2699489758583298No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCACA207.004258E-445.012312
AGTGTAC207.0157554E-444.99733728
TGCCGTC24500.044.17085645
TAATAGT250.002098476536.0098534
CTAAGAG250.002098476536.0098534
GCTAGAA250.002101908435.9978732
GCCGTCT401.552222E-533.74845
GTTTGAG300.005134684529.99822436
ATGCCGT37400.028.87529844
TATGCCG39500.027.39711243
GTATGCC39600.027.32792942
TCTCGTA39700.027.31576738
CTCGTAT39800.027.30366339
ATCTCGT39900.027.23523337
GCCCACG40100.027.21160718
CGTATGC40050.027.18940441
TATCTCG39850.027.15648736
CCTATCT39850.027.15648734
TCGTATG40100.027.155540
CCACGAG40200.027.14391720
Produced by FastQC (version 0.11.2)