FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pa_54.35100000043df6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pa_54.35100000043df6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217036
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC12447857.353618754492345No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT4176319.242429827309753No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCG117805.427670985458634No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAAC253.8803177E-545.028
ACGAGAT207.02063E-445.022
AGACGAC253.8803177E-545.025
GACGACA253.8803177E-545.026
CGAGATG207.02063E-445.023
TCCCGAG207.02063E-445.013
TGCCGTC129350.044.65210745
GCCGTCT950.040.26315745
CAACCTA403.440964E-739.37531
GACAACC301.1368729E-437.49999629
CACTCCG301.1368729E-437.4999969
CCTATCC250.002103352236.034
AACTCGT250.002103352236.037
ATGCCGT172850.033.40179444
CACACTC352.8128922E-432.1428577
ACACTCC352.8128922E-432.1428578
ACTCCGA352.8128922E-432.14285710
ACGACAA352.8128922E-432.14285727
TATGCCG184900.031.23715443
ATCTCGT185200.031.23515137
Produced by FastQC (version 0.11.2)