FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pa_53.35100000043ddc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pa_53.35100000043ddc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146562
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC6738545.97712913306314No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT101636.934266726709515No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCG43772.9864494207229706No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAGAG207.0133974E-444.999667
TGCCGTC69900.044.5490245
GCCGTCT700.041.78539745
ATGCCGT80100.038.84802244
GTATTGG301.13283975E-437.5125121
TATGCCG85000.036.60854743
CGAGCCC85500.036.6049915
GCCCACG85450.036.60007518
AGCCCAC85450.036.60007517
GAGCCCA85450.036.60007516
ATCTCGT85400.036.59515837
TAACCTA85250.036.58036431
TATCTCG85400.036.56881336
TCTCGTA85400.036.56881338
CGAGACG85650.036.56714623
CTCGTAT85350.036.5638739
CACGAGA85800.036.55566821
CCACGAG85750.036.55074320
GTATGCC85200.036.5490242
GAGACGA85700.036.54581524
Produced by FastQC (version 0.11.2)