FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pa_52.35100000043db2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pa_52.35100000043db2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences275026
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC79362.8855453666198834No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT30911.1238937409554006No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCG5920.2152523761389832No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC4280.15562165031669734No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT3100.1127166158835892No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC2850.1036265662155578No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA2780.10108135230850901No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC7900.043.5736645
CTAGGAC250.002101115236.0112083
TAGGACA401.5529562E-533.76054
ACCTATA352.8067012E-432.158711
CTGTCCA352.8157042E-432.141179
CGGGACA352.8157042E-432.1411717
ATGCCGT10900.031.5809144
GTGTAAT453.4695906E-530.0147971
CGTATGC12150.028.33184441
TATGCCG12150.028.1466743
CTCGTAT12250.028.10056539
TATCTCG12250.028.10056536
TCGTATG12250.028.10056540
CGAGACG12200.028.03131323
GTATGCC12250.027.91690342
CCTATCT12450.027.82986334
TCTCGTA12500.027.71854638
ATCTCGT12500.027.53855537
ACGAGAC12500.027.53855522
AACCTAT12450.027.46843532