FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pa_46.35100000043d05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pa_46.35100000043d05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences234833
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC4756420.254393547755214RNA PCR Primer, Index 46 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGT2869512.219321815928765No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCG48742.075517495411633No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGC3350.14265456728824313No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGTC207.015264E-445.008523
ACACGGA207.022631E-444.99893618
ACCGATC207.022631E-444.99893629
TGAACCG207.022631E-444.99893640
CTGCGGT207.022631E-444.99893625
GCCGAAA253.881838E-544.99893611
CCATCCA207.022631E-444.99893610
CGGTGAT207.022631E-444.99893622
ACGTCGT207.022631E-444.9989366
ATGAATC207.022631E-444.99893614
CTCGAGG207.022631E-444.99893618
CACGTAG207.022631E-444.99893618
GCCGTCT406.7775545E-944.99893645
TACACGG207.022631E-444.99893617
TGCCGTC48550.044.58185245
ACTGGAC301.1358901E-437.50713
GACAGAT301.1373157E-437.4991157
CCAGAAG301.1373157E-437.49911518
TCCAGGC301.1373157E-437.49911525
CTACTGG250.00210174836.0068171
Produced by FastQC (version 0.11.2)