FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pa_45.35100000043cea.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pa_45.35100000043cea.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165242
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC9564957.88419409109065RNA PCR Primer, Index 46 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGT2845017.21717238958618No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCG66614.031057479333342No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCA207.0069043E-445.0115055
GCCCACA207.017367E-444.99788318
TTCCGAT207.017367E-444.99788331
ACACTCC207.017367E-444.9978838
ACTCTGG207.017367E-444.99788311
TGCAGTC302.1548894E-644.9978845
TGCCGTC97550.044.4212845
GCCGTCT1200.043.1229745
TCCTAAT301.13601076E-437.49823432
TAATCTC301.13601076E-437.49823435
AGTACTC250.002099264236.0092055
CACTCCG250.002102387135.9983069
ATGCCGT127750.033.92012844
TCCGAGC136050.032.16496713
CTAATCT352.810778E-432.14134634
TCTCCGA136200.032.12954311
CTCCGAG136200.032.12954312
CCGAGCC136300.032.1224814
AGCCCAC136600.032.1013417
CGAGCCC136600.032.1013415
Produced by FastQC (version 0.11.2)