FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pa_40.35100000043c3d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pa_40.35100000043c3d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182555
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC2741815.019035359206814No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT2278012.478431157733286No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCG35521.9457149900030126No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAT207.017685E-445.00000430
ACCTAGT207.017685E-445.0000041
CTTAGCT207.017685E-445.0000049
TGCCGTC28900.043.9878945
ACGTATG301.13616436E-437.49999614
CACGTAT301.13616436E-437.49999613
TATACTG301.13616436E-437.4999965
AACCGGA250.002102478636.019
ACCGGAA250.002102478636.020
ATGCGTA250.002102478636.05
CACGTTA250.002102478636.029
TAGGAGC250.002102478636.010
CCTATGC250.002102478636.03
GCACTAA250.002102478636.028
GCGTAAC250.002102478636.07
TGCGTAA250.002102478636.06
TCCTATG352.8111524E-432.1428572
TTACATA352.8111524E-432.1428572
ACACGTA300.00513605629.99999812
CATATAG300.00513605629.9999985
Produced by FastQC (version 0.11.2)